N^+离子注入筛选高产色素的红曲菌株及其发酵条件的研究  

Mutation breeding of Monascus pigments strain by N^+ ion implantation

作　　者：秦红霞[1] 周立平[1] 嘉晓勤[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《中国酿造》2011年第4期68-71,共4页China Brewing

基　　金：浙江省教育厅科研项目(Y200908192)

摘　　要：以色素产生菌紫色红曲菌M5(Monascus purpureus)为出发菌株,运用N+离子束注入对其进行诱变选育,当诱变的能量为10keV,剂量为1.56×1015ions/cm2时,致死率为71.2%,经平板分离纯化和摇瓶筛选,获得一株突菌株M532,其液态发酵摇瓶的红色素达336U/mL,黄色素达321U/mL,相比于原菌株提高了121%,经多次传代稳定性试验证明其发酵传代稳定性良好;在5L的发酵罐中进行放大实验,红色素的产量为238U/mL,黄色素为224U/mL。以大米作培养基,其固态发酵的红色素产量达到7620U/g,黄色素达6590U/g。In order to obtain an industrial strain with higher Monascus pigments yield,the strain Monascus purpureus M5 was mutated by N＋ implantation.With energy of 10keV and dose of 1.56×1015ions/cm2,the lethality rate was 71.2%.One mutant strain,M532 with high yield of pigments was isolated,and red pigment production was increased from 154U/ml to 336U/ml and yellow pigment from 141U/ml to 321U/ml in the shaking flask culture.Moreover,the mutant strain inheritance and fermentation stability was stable.The scale up test was conducted in a 5L fermenter,and the red pigment and yellow pigment reached 238U/ml was 224U/ml,respectively.Using rice as solid substrate,the yield of red pigment and yellow pigment was 7620U/g and 6590U/g,respectively.

关 键 词：红曲色素 N+离子注入 发酵 

分 类 号：TS201.3[轻工技术与工程—食品科学]