4种蜂花粉油脂抗氧化活性研究  

Antioxidant Activity of Four Kinds of Bee Pollen Oil

作　　者：何伟[1,2] 徐响[1] 孙丽萍[1] 庞杰[2] 穆雪峰[1,2] 黄兰[1,2] 沈新锋[1,2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]福建农林大学食品科学学院,福建福州350002

出　　处：《食品科学》2011年第17期114-117,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD33B04);科技部农业科技成果转化资金项目(2009GB23260456);国家现代农业(蜂)产业技术体系建设专项(CARS-45-KXJ18)

摘　　要：采用ABTS.+清除、DPPH自由基清除、磷钼络合物法3种体外抗氧化方法,以天然抗氧化剂VE(α-生育酚)作为对照,评价并比较五味子、芝麻、玉米和蚕豆蜂花粉油脂的抗氧化活性。结果表明:4种蜂花粉油脂对ABTS.+和DPPH自由基均具有清除能力,但清除能力存在极显著性差异(P<0.01),IC50值分别在0.029～0.708mg/mL和0.059～2.203mg/mL之间;在磷钼络合物法中,抗氧化活性为五味子>玉米、蚕豆>芝麻。在3种抗氧化方法中五味子蜂花粉油脂的抗氧化能力最强,与天然抗氧化剂VE相当。The antioxidant activities of Fructus Schisandrae,sesame,corn and bean bee pollen oils were evaluated by DPPH radical scavenging method,ABTS radical scavenging method and phosphomolybdenum method using α-tocopherol as the positive control.Results indicated that four kinds of bee pollen oil had strong scavenging effect on ABTS and DPPH free radicals with IC50 values of 0.029-0.708 mg/mL and 0.059-2.203 mg/mL,respectively.Moreover,their scavenging activities against DPPH and ABTS free radicals revealed a significant difference（P 0.01）.Through determining by phosphomolybdenum method,the strongest antioxidant activity was Fructus Schisandrae bee pollen oil,followed by corn,bean and sesame bee pollen oils.Moreover,Fructus Schisandrae bee pollen oil revealed antioxidant activity equivalent to that of α-tocopherol.

关 键 词：蜂花粉油脂 抗氧化活性 2 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS) 1 1-二苯基-2-三硝基苯肼(DPPH) 磷钼络合物法 

分 类 号：TS201.2[轻工技术与工程—食品科学]