皂荚多糖胶酶解制备低聚糖  

Preparation of Oligosaccharides from Polysaccharide Gum of Gleditsia sinensis by Enzymatic Hydrolysis

作　　者：杨洋[1] 菅红磊[1] 徐永霞[1] 朱莉伟[1] 

机构地区：[1]北京林业大学材料科学与技术学院,北京100083

出　　处：《食品科学》2011年第18期138-141,共4页Food Science

基　　金：国家自然科学基金面上基金项目(30771685);北京林业大学大学生科研训练计划项目(100504)

摘　　要：利用β-甘露聚糖酶对皂荚多糖胶进行解聚改性。采用L9(34)正交设计法对底物质量浓度、加酶量、反应温度和反应时间4个因素进行考察。正交试验结果表明:皂荚多糖胶酶解的最佳工艺条件为底物质量浓度50g/L、加酶量1300U/g、反应温度65℃、反应时间11h。在此最佳工艺条件下,还原性末端糖基得率为49.92%,酶解产物的平均聚合度为2.00。此外,通过初期补料方式提高底物浓度,在反应24h后100g/L和150g/L的反应液中还原性末端糖基得率分别为50.89%和46.97%。表面活性剂对酶解反应有促进效果,皂荚皂素和吐温80可将还原性末端糖基得率分别相对提高5.25%和9.40%。高效液相色谱分析表明,皂荚多糖胶的酶解产物主要为甘露四糖(17.25%)、甘露三糖(28.68%)、甘露二糖(4.55%),水解产生的单糖仅为1.81%。The depolymerization of gum derived from Gleditsia sinensis was performed by enzymatic hydrolysis with β-mannanase.The optimal hydrolysis conditions were investigated by using an L9（34） orthogonal array design to explore the effects of substrate concentration,enzyme dosage,hydrolysis time and hydrolysis temperature on average polymerization degree of hydrolysates and reducing end glycosyl group yield.The results showed that the optimal conditions for depolymerization of the gum were substrate concentration of 50 g/L,enzyme dosage of 1300 U/g,reaction temperature of 65 ℃,and reaction time of 11 h.Under the optimal hydrolysis conditions,the reducing end glycosyl group yield was 49.92% and the average degree of polymerization 2.00.In addition,fed batch operation at the beginning could increase substrate concentration,and he reducing end glycosyl group yields in 100 g/L and 150 g/L reaction solutions after reaction for 24 h were 50.89% and 46.97%,respectively.The addition of surfactants such as gleditsia saponin and T-ween 80 could improve reducing end glycosyl group yield by 5.25% and 9.40%,respectively.Moreover,HPLC analysis indicated that the hydrolysates of Gleditsia sinensis gum mainly consisted of mannotetraose（17.25%）,mannotriose（28.68%）,mannobiose（4.55%） and monosaccharide（1.81%）.

关 键 词：皂荚多糖胶 酶解 还原性末端糖基 正交试验 低聚糖 

分 类 号：TQ432.9[化学工程]