油菜、菊花和荷花蜂花粉中磷脂的色谱分析  

Chromatographic Analysis of Phospholipids in Rape,Chrysanthemum,and Lotus Bee Pollens

作　　者：何伟[1,2] 徐响[1] 孙丽萍[1] 庞杰[2] 黄兰[1,2] 穆雪峰[1,2] 沈新锋[1,2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]福建农林大学食品科学学院,福建福州350002

出　　处：《食品科学》2011年第18期185-189,共5页Food Science

基　　金："十一五"国家科技支撑计划项目(2011BAD33B04);科技部农业科技成果转化项目(2009GB23260456);国家现代农业(蜂)产业技术体系建设专项(CARS-45-KXJ18)

摘　　要：以油菜、菊花、荷花蜂花粉为原料,采用薄层层析法(thin layer chromatography,TLC)法分离纯化蜂花粉中的磷脂,并用高效液相色谱法(high-performance liquid chromatography,HPLC)法测定磷脂酰肌醇(PI)、磷脂酰丝氨酸(PS)、脑磷脂(PE)、卵磷脂(PC)及溶血卵磷脂(LPC)的含量。结果表明:3种蜂花粉中总磷脂含量为1.19～3.98g/100g,3种花粉存在极显著差异(P<0.01);PC是蜂花粉磷脂的主要成分,占总磷脂的34.30%～59.69%;在油菜蜂花粉中检测到PI、PS、PE、PC、LPC,菊花蜂花粉未测出PI,荷花蜂花粉未测出PI、LPC。结论:油菜蜂花粉中磷脂种类最丰富、总含量最高,是花粉磷脂的较好来源。The phospholipids in rape,chrysanthemum,and lotus bee pollens were separated and purified by TLC,and were quantitatively determined by HPLC.The results indicated that the total phospholipid contents in three bee pollens were from 1.19 to 3.98 g/100 g with a significant difference（P0.01）.Phosphatidylchuline（PC） was the predominant phospholipid in bee pollen,representing 34.30%-59.69% of total phospholipids.Phosphatidylinositol（PI）,phosphatidylserine（PS）,phosphatidyl ethanolamine（PE）,phosphatidylcholine（PC） and lysophosphatidylcholine（LPC） were detected in rape bee pollen,but no PI in chrysanthemum bee pollen,and neither PI nor LPC in lotus bee pollen.As a conclusion,rape bee pollen contains the most phospholipids with the largest total content among the three sources investigated.

关 键 词：蜂花粉 高效液相色谱法(HPLC) 磷脂 含量 

分 类 号：TS201.2[轻工技术与工程—食品科学]