高效液相色谱-串联质谱法测食品中的赭曲霉毒素A  

Analysis of Ochratoxin A in Foods by HPLC-MS/MS

作　　者：史娜[1] 路勇[1] 吴颖[2] 姜杰[1] 

机构地区：[1]北京市食品安全监控中心,北京100041 [2]北京市产品质量监督检验所,北京100026

出　　处：《食品科学》2011年第18期260-263,共4页Food Science

摘　　要：研究建立高效液相色谱-串联质谱联用技术检测食品中赭曲霉毒素A的方法。根据不同样品,用甲醇-2%碳酸氢钠溶液(60:40,V/V)或甲醇-水(80:20,V/V)提取样品中的赭曲霉毒素A,经OchraTest亲和柱净化,以甲醇-5mmol/L(含0.1%甲酸)乙酸铵为流动相,采用正离子模式对赭曲霉毒素A进行检测。结果回收率在82.3%～98.5%之间,检出限为0.1μg/kg。该方法适用不同基质样品,准确性好、灵敏度高、抗干扰能力强,方法检出限可满足欧盟等最新限量要求。A high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） method was proposed to determine ochratoxin A（OTA） in foods.Samples were extracted by methanol-2% sodium bicarbonate solution（60：40,V/V） or methanol-water（80：20,V/V）,followed by clean-up on OchraTest affinity cartridge using methanol-5 mmol/L ammonium acetate solution in the presence of 0.1% formic acid as the mobile phase.OTA was detected in the in positive ion mode.The average spike recoveries for ochratoxin A in 4 food samples were 82.3%-98.5%（n =5）.The limit of detection was 0.1 μg/kg,which can meet the latest requirement of the EU.Moreover,this method proved suitable for a broad range of sample matrices with high accuracy,sensitivity and disturb resistance.

关 键 词：高效液相色谱-串联质谱 赭曲霉毒素A 免疫亲和柱 多离子反应监测 

分 类 号：TS207.3[轻工技术与工程—食品科学]