康宁木霉固态发酵生产纤维素酶与木聚糖酶的研究  

Solid State Fermentation for Cellulase & Xylanlase Production by Trichoderma Koningii

作　　者：邹水洋[1] 刘传生[1] 朱丹[1] 

机构地区：[1]东莞理工学院化学与环境工程学院,广东东莞523808

出　　处：《东莞理工学院学报》2011年第5期55-59,共5页Journal of Dongguan University of Technology

基　　金：东莞市科技计划项目(2008108101013)

摘　　要：以稻草和麸皮为主要原料,采用康宁木霉QF-02固态发酵生产纤维素酶及木聚糖酶。对影响产酶的多个因素进行了研究,获得了如下优化培养条件:稻草粉与麸皮比为3∶2(w/w),原料粒径为20目,料水比为1∶1.5(w/v),起始pH值5.5,28.5℃培养时间120 h。菌种在此优化培养条件下的滤纸酶活、β-葡萄糖苷酶活、木聚糖酶活分别达到13.1 IU/g(干曲)、12.2 IU/g(干曲)h和994.7 IU/g(干曲)。Cellulase and Xylanase was produced under solid state fermentation by Trichoderma koningii QF-02 using rice straw and wheat bran as main substrate. A series of factors affecting on the synthesis of enzymes were investigated and then the optimal culture condition was set as follows： straw to bran ratio of 3：2 （w/w） , solid materials to Mandels nutritive solution ratio of 1 ： 1.5 （ w/v）, initial pH at 5.5 and cultivation for 120 h at 28.5 %. Under the optimal culture condition, the enzymatic activity of filter paper cellulase,

关 键 词：康宁木霉 纤维素酶 木聚糖酶 固态发酵 

分 类 号：Q556.2[生物学—生物化学]