红曲多糖液态发酵条件与抗氧化活性的研究  

Optimization of Liquid-Fermentation Conditions of Monascus Exopolysaccharides(MEP) and Its Antioxidant Activity

作　　者：汪鹏荣[1] 陈攀攀[1] 蓝丽精 蒋冬花[1] 林遥雪[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《微生物学杂志》2011年第4期19-24,共6页Journal of Microbiology

基　　金：国家自然科学基金(31070008);浙江省大学生新苗人才计划项目(ZC323010037)

摘　　要：对1株高产胞外多糖红曲霉菌株Mr-70的液态发酵条件和粗多糖抗氧化活性进行了初步研究,得到菌株Mr-70优化后的发酵工艺条件为葡萄糖60 g/L,蛋白胨20 g/L,K2HPO410 g/L,MgSO4.7H2O 1.1 g/L,pH6.5,在摇床转速200 r/min,温度32℃的条件下培养96 h,胞外粗多糖产量可达10.15 g/L;5 mg/mL的红曲霉胞外粗多糖的DPPH.清除率为82.24%,表现出较高的抗氧化活性。Liquid-fermentation conditions of a high MEP yielding Monascus strain Mr-70 and antioxidant activity of its crude MEP were initially studied.The fermentation conditions were optimized as follows： glucose 60.0 g/L,peptone 20.0 g/L,KH2PO4 10.0 g/L,MgSO4·7H2O 1.1 g/L,pH 6.5,shaker at 200 r/min,cultured at 32 ℃ for 96 h.Under these conditions,the yield of MEP reached as much as 10.15 g/L in shaker flask.The scavenging rate of crude MEP adopting DPPH· was 82.24% within 5 mg/mL,manifested that MEP of Monascus Mr-70 had fairly strong antioxidant activity.

关 键 词：真菌多糖 红曲霉 胞外多糖 抗氧化活性 

分 类 号：Q939.9[生物学—微生物学]