不同酶酶解鸡胚蛋白工艺比较研究  

Comparative Studies of Enzymatic Hydrolysis of Chicken Embryo Protein by Different Proteases

作　　者：梁琼[1] 张培正[2] 高伟[2] 孙合美[2] 

机构地区：[1]海口经济学院工程技术学院,海南海口570203 [2]云南农业大学食品科学与工程学院,云南昆明650201

出　　处：《肉类研究》2011年第9期21-24,共4页Meat Research

基　　金：海南省自然科学基金项目(310058)

摘　　要：以鸡胚蛋白为底物,通过单因素及正交试验,确定菠萝蛋白酶和胰蛋白酶对鸡胚蛋白水解的最佳工艺。结果表明:菠萝蛋白酶酶解鸡胚蛋白最佳工艺条件为底物质量分数6%、酶解温度55℃、pH5、酶解时间6h、酶与底物质量比([E/S])为1.5%,水解度可达45.53%;胰蛋白酶酶解鸡胚蛋白最佳工艺条件为底物质量分数6%、酶解温度55℃、pH5、酶解时间6h、酶与底物质量比([E/S])为1.5%,水解度可达38.17%。菠萝蛋白酶和胰蛋白酶在最佳水解条件下水解度分别为45.53%、38.17%,可溶性蛋白含量分别为20.15、22.69mg/mL,多肽含量分别为62.55、50.26mg/mL。菠萝蛋白酶比胰蛋白酶更适宜酶解鸡胚蛋白。The optimal process conditions for hydrolyzing chicken embryo protein by trypsin or bromelain were determined using one-factor-at-a-time combined with orthogonal array design method. Under the optimal process conditions, the degree of hydrolysis of chicken embryo protein by trypsin and bromelain were 45.53% and 38.17%, and the resulting hydrolyates contained 20.15 mg/mL and 22.69 mg/mL soluble protein and 62.55 mg/mL and 50.26 mg/mL polypeptides, respectively. These results demonstrate that bromelain is more suitable to hydrolyze chicken embryo protein than trypsin.

关 键 词：鸡胚 蛋白酶 酶解 多肽 

分 类 号：TS201.21[轻工技术与工程—食品科学]