高富硒酵母菌种选育研究  

Breeding of a High Selenium-enriched Yeast Strain

作　　者：王小波[1] 柳乐[1] 李学如[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《食品与发酵科技》2011年第5期6-9,共4页Food and Fermentation Science & Technology

基　　金：中央高校基本科研业务专项资金(SWJTU11ZT25)资助项目

摘　　要：以富硒酵母Y2为出发菌株,通过60Coγ射线照射诱变育种,筛选获得了一株生物量和富硒能力都较高的突变菌株YR166。通过正交实验确定变异株最适发酵培养基配方为:麦芽汁10%,(NH4)2SO4 1.0%,KH2PO4 0.2%,Se4+浓度为50μg/mL。在培养温度30℃,500mL三角瓶装量60mL,摇瓶培养6h加硒无机硒后,继续摇瓶培养30h,酵母硒含量达到2531.4μg/g,较原菌株提高了74.2%,酵母生物量达到14.9g/L,较原菌株提高36.7%。A yeast strain Y2 was mutagenized by the radial of 60Co-γ.A high selenium-enriched strain YR166 was screened from mutant strains of Y2.The compositions of culture medium for the growing of YR166 was gained by a L9（34） orthogonal test,which are 10% of wort,1.0% of（NH4）2SO4,0.2% of KH2PO4,and 50 μg/mL of the inorganic Se4＋.The optimal conditions of fermentation for YR166 strain were the liquid volume of 60 mL in 500 mL erlenmeyer flask for 30h.Se was added in the culture at 6h.The cultivation temperature was 30℃.Under the optimized conditions the total Se content of the YR166 cells reached 2531.4μg/g,which was 74.2% higher than that of Y2 and the biomass（dry weight） was 14.9g/L,36.7% higher than that of Y2.

关 键 词：富硒酵母 诱变育种 发酵工艺 

分 类 号：TQ926.1[轻工技术与工程—发酵工程]