响应面法优化植酸酶水解大豆分离蛋白的工艺  

Optimization of phytase hydrolysis of soybean protein isolated by response surface methodology

作　　者：张仁楠[1] 姚磊[2] 富校轶[1,2] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]国家大豆工程技术研究中心,黑龙江哈尔滨150030

出　　处：《食品工业科技》2011年第11期292-295,共4页Science and Technology of Food Industry

基　　金：国家"863"计划项目(2006AA10Z332);黑龙江省青年基金项目(41400075-6-08003)

摘　　要：以大豆分离蛋白为实验材料,在酶解温度、酶添加量、pH、水解时间等单因素实验的基础上,根据Box-Behnken中心组合实验设计原理,采用四因素三水平响应曲面分析法,依据回归分析确定各工艺条件的主要影响因素,以大豆分离蛋白中植酸的去除率为响应值作响应面。植酸去除的最佳工艺条件为:反应温度44.87℃,酶添加量为8.24%,pH为5.54,反应时间为3.03h。在最佳工艺条件下,植酸的去除率为95.15%,验证实验结果为94.98%。With soybean protein isolated as experiment material,on the base of single factor experiments（hydrolysis time,hydrolysis temperature and so on）,then through the experimental design of response surface methodology with four factors and three levels based on the principle of Box-Behnken design,the conditions of hydrolysis were studied.The optimal hydrolysis conditions：hydrolysis temperature was 44.87℃,exterior phytase addition about 8.24%,pH was 5.54,hydrolysis time was 3.03h.Experiments that checked the optimal hydrolysis time conditions showed that phytate of soybean protein isolated was degraded about 95.15%,proof test result was 94.98%.

关 键 词：大豆分离蛋白 植酸 植酸酶 响应面 

分 类 号：TS201.21[轻工技术与工程—食品科学]