鲍鱼脏器糖蛋白超声辅助提取工艺及分离纯化的研究  

Study on the Ultrasound-assisted Extraction and Purification of Abalone Visceral Glycoprotein

作　　者：包郁明[1] 陶宇[1] 侯虎[1] 赵雪[1] 李八方[1] 

机构地区：[1]中国海洋大学食品与工程学院

出　　处：《食品与发酵工业》2011年第9期11-15,共5页Food and Fermentation Industries

基　　金：国家自然科学基金(30871943);国家自然科学基金(30800858)

摘　　要：以冻藏鲍鱼脏器为试验原料,分析了鲍鱼脏器的基本成分组成,研究了鲍鱼脏器糖蛋白的分离纯化及纯化物基本组成。通过单因素试验、正交试验确定了最适提取条件为:5%NaCl溶液、料液质量比1∶9、超声功率为300 W、超声处理时间为40 min,在此条件下,糖蛋白提取率为69.7 mg/g(干重),比传统方法提高了28.5%。糖蛋白粗品经硫酸铵沉淀,DEAE-Cellulous 52柱层析,SephacrylS-300 HR柱层析后,经SDS-PAGE电泳鉴定得到分子质量为73.7 ku的组成均一的糖蛋白AGP-Ⅱ,AGP-Ⅱ含有总糖12.4%,总蛋白76.6%。Frozen abalone visceral were used as materials. The lyzed. The optimized conditions of ultrasound-assisted glycoprotein general components of abalone visceral were anaextraction was selected by orthogonal analysis on the basis of single factor experiments. The optimized conditions of ultrasound-assisted extraction were as follows ： NaCl concentration was 5 % ,the ratio of material to solvent was 1 ： 9, ultrasonic power was 300 W and ultrasonic time was 40 min. The extraction rate of glycoprotein in the extraction was 69.7mg/g by this method, the extraction ratio increased by 28. 5% compared to the tradition method. Furthermore, the glycoproteins were precipitated by （NH4）2SO4 and isolated by DEAE-Cellulose and SephacrylS-300 HR. Results showed that a homogeneous glycoprotein （ AGP- Ⅱ ） was obtained. The molecular weight of AGP-Ⅱ was estimated to be 73.7 ku by SDS-PAGE. AGP- Ⅱ contained 12.4% total sugar, 76.6% protein.

关 键 词：鲍鱼脏器 糖蛋白 超声提取 分级层析 纯化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]