LAMP法在阪崎肠杆菌快速检测中的应用  

The application of loop mediated isothermal amplification(LAMP)technique for rapid detection of Ent erob a cter sakazakii

作　　者：张淑红[1,2,3,4] 吴清平[4] 徐晓可[1,2,3,4] 张菊梅[4] 郭伟鹏[4] 

机构地区：[1]广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地,广东广州510070 [2]广东省菌种保藏与应用重点实验室,广东广州510070 [3]广东省微生物应用新技术公共实验室,广东广州510070 [4]广东省微生物研究所,广东广州510070

出　　处：《食品与机械》2011年第5期111-114,共4页Food and Machinery

基　　金：广东省科技计划项目(编号:2008A040101001;2009B040500001);广东省科学院青年科学研究基金项目(编号:qnjj20099)

摘　　要：针对阪崎肠杆菌的ompA基因设计4条特异性引物(2条内引物、2条外引物),建立一种快速检测阪崎肠杆菌的DNA环介导恒温扩增法(LAMP)。通过对2株阪崎肠杆菌标准菌株、24株阪崎肠杆菌分离株和18株非阪崎肠杆菌测试,结果表明此方法具有很高的特异性;对其灵敏度进行检验,发现该方法的最低检测限可以达到8CFU/mL,具有较好的敏感性。在1份实验室能力验证盲样和26份实际奶粉样品中应用发现,该方法与传统生化方法结果吻合,具有较好的可靠性。Four primers including two inner primers,two outer prim-ers were designed specifically to recognize the ompA gene of E.sakazakii and a method of loop-mediated isothermal amplification was set up to detect Enterobacter sakazakii rapidly.The results sug-gested that the method had high speciality to 2 E.sakazakii typical strains,24isolates and 18non-E.sakazakii.More over,it had good sensitivity with the lowest detectable limit of 8CFU/mL.Through detecting the proficiency tests of E.sakazakii in milk pow-der and 26milk powder samples by this method,the results indicated that it had the same results as traditional biochemical method and may be used to detect samples.

关 键 词：阪崎肠杆菌 ompA基因 环介导恒温扩增方法 

分 类 号：TS207.4[轻工技术与工程—食品科学]