苦荞多糖的分离纯化及单糖组成测定  

Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides

作　　者：颜军[1] 孙晓春[2] 谢贞建[1] 何钢[1] 易勇[1] 房晨[1] 苟小军[1,2] 

机构地区：[1]成都大学中药化学实验室,四川成都610106 [2]四川抗菌素工业研究所,四川成都610052

出　　处：《食品科学》2011年第19期33-36,共4页Food Science

基　　金：四川省中医药管理局科技专项资助项目(2008-01);四川省教育厅科研项目资助项目(08ZA173);成都大学校基金项目(2010XJZ24)

摘　　要：采用水提醇沉法获得苦荞多糖,经离子交换柱分离,高效凝胶过滤色谱法(HPGFC)纯度鉴定并测定多糖的相对分子质量。硫酸水解后,用1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生水解后的单糖,衍生物采用反相高效液相色谱法测定单糖组成。结果表明:苦荞经水提醇沉,用Sevag法脱蛋白后,获得苦荞多糖(TBP);DEAE-纤维素柱层析获得3个苦荞多糖组分TBP-1、TBP-2和TBP-3,相对分子质量分别为144544、445656和636795。柱前衍生液相色谱分析可知:TBP-1、TBP-2是由葡萄糖组成的均一多糖;TBP-3由甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成的杂多糖,其物质的量比为4.32:2.41:1.00:39.8:9.64:2.02。Tartary buckwheat polysaccharides（TBPs） were extracted by boiling water,precipitated by alcohol and purified by ion-exchange column chromatography.The purity and molecular weight of TBPs were determined by high performance gel filtration chromatography（HPGFC）.Polysaccharide samples were hydrolyzed with sulfuric acid solution,and derivatized with 1-phenyl-3-methyl-5-pyrazolone（PMP）.The monosaccharide composition of the hydrolysates obtained was analyzed by RP-HPLC.The results showed that TBPs could be obtained by water extraction and alcohol precipitation after protein removal by Savag method.Three polysaccharides,named as TBP-1,TBP-2 and TBP-3,were obtained by DEAE-cellulose column chromatography with molecular weights of 144544,445656 and 636795,respectively.Liquid chromatographic analysis based on pre-column derivatization showed that TBP-1 and TBP-2 were composed of glucose and TBP-3 of mannose,rhamnose,glucuronic acid,glucose,galactose and arabinose in a molar ratio of 4.32：2.41：1.00：39.8：9.64：2.02.

关 键 词：苦荞 多糖 单糖 衍生化 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]