β-葡聚糖酶活力测定方法的研究进展  

Research Progress of Determination Methods for β-Glucanase Activity

作　　者：张永勤[1] 曾凡伟[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《食品科学》2011年第19期316-320,共5页Food Science

摘　　要：本文综述了β-葡聚糖酶活力的测定方法,如黏度法、生色底物法、琼脂糖扩散法、酶联免疫吸附分析法(ELISA)和目前使用较多的还原端基法,如3,5-二硝基水杨酸(DNS)法、Somogyi-Nelson法、二金鸡纳酸(BCA)法和最新涌现的用于微量测定的3-甲基-2-苯并噻唑酮腙(MBTH)法,并就还原端基法介绍了其具体实验手段,如试管法、罐法、微孔板法、流动注射法(FIA)。分析了方法中存在的问题并展望了β-葡聚糖酶活力测定方法研究的发展趋势,如安培法和等温滴定量热法等。In this paper,the present determination methods for β-glucanase activity are reviewed,which includes viscometric method,chromomeric method,agar diffusion method,and commonly used end-reducing methods such as DNS,Somogyi-Nelson and BCA methods,as well as novel MBTH method.In addition,the end-reducing methods are classified as end-point method,multiple-point method,microplate method,and flow injection analytic method according to the operation procedures.Meanwhile,current problems with these methods are discussed,and the future development trends of the determination methods are proposed.

关 键 词：Β-葡聚糖酶 测定 DNS法 MBTH法 微孔板法 流动注射法 试管法 罐法 

分 类 号：TS262.5[轻工技术与工程—发酵工程]