不同相对湿度对板栗“石灰化”及活性氧代谢的影响  

Effect of Different Levels of Relative Humidity on Calcification and Reactive Oxygen Metabolism of Chinese Chestnuts

作　　者：顾采琴[1] 张颖豪[2] 张倩[2] 朱冬雪[1] 郑志茂[1] 邱浩纯[1] 

机构地区：[1]广州大学化学化工学院,广东广州510006 [2]贵州大学生命科学学院,贵州贵阳550025

出　　处：《食品科学》2011年第20期296-299,共4页Food Science

基　　金：国家自然科学基金面上项目(30871760)

摘　　要：以新鲜板栗为试材,研究在不同相对湿度(50%、70%、90%)条件下进行常温(25℃)贮藏过程中,板栗的水分含量、"石灰化"指数、超氧阴离子自由基(O-2.)产生速率、过氧化氢(H2O2)含量、超氧化物歧化酶(superoxidase dimutase,SOD)和过氧化氢酶(catalase,CAT)活性及膜脂过氧化产物丙二醛(malondialdehyde,MDA)含量的变化,以探讨不同相对湿度对板栗"石灰化"及活性氧代谢的影响。结果表明:在高湿(90%)条件下贮藏的板栗水分含量的减少和"石灰化"指数均受到显著抑制,O-2.生成速率、H2O2含量和MDA含量均极显著低于在低湿(50%)条件贮藏的板栗,而SOD活性、CAT活性则极显著高于在低湿条件下贮藏的板栗。表明板栗采后贮藏在高湿环境中能有效控制"石灰化"发生,维持较高活性氧清除能力,使板栗过氧化作用较低。In order to understand the effects of relative humidity on calcification and reactive oxygen species metabolism of Chinese chestnuts,the changes of moisture,calcification index,superoxide anion free radical（O2-·） production rate,H2O2 content,SOD and CAT activities,and the content of MDA（malondialdehyde） of Chinese chestnut stored under different levels of relative humidity such as 50%,70% and 90% and room temperature（25 ℃） were determined.The results showed water loss and calcification index of Chinese chestnuts stored under high relative humidity（90%） were significantly decreased.Meanwhile,O2-· production rate,H2O2 and MDA content were significantly lower,while SOD and CAT activities of chestnuts were significantly higher as compared with Chinese chestnuts stored under low relative humidity（50%）.These findings reveal that high relative humidity（90%） efficiently inhibits the occurrence of calcification,maintains higher reactive oxygen species scavenging activity and decrease lipid peroxidation in Chinese chestnuts during postharvest storage.

关 键 词：相对湿度 板栗 “石灰化” 活性氧代谢 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]