高效液相色谱法同时测定糯米酒中17种氨基酸  

Simultaneous Determination of 17 Kinds of Amino Acids Content in Yellow Rice Wine by RP-HPLC with Post-column Derivatization

作　　者：曾玩娴[1] 刘天质[1] 

机构地区：[1]广东省酒类检测中心,广东广州510405

出　　处：《酿酒科技》2011年第11期120-121,共2页Liquor-Making Science & Technology

摘　　要：建立反相高效液相色谱法(RP-HPLC)用以测定糯米酒中的17种氨基酸。采用OPA试剂柱后衍生,RP-HPLC经梯度洗脱后检测糯米酒中17种氨基酸的含量。结果表明,该方法测定17种氨基酸的线性相关系数R在0.9990～0.9998,平均回收率为94.5%～98.7%,精密度RSD为0.51%～1.90%。该方法专属性强、操作方便、灵敏度高,可用于糯米酒和花雕酒等黄酒中17种氨基酸含量的检测。RP-HPLC has been developed for simultaneous determination of 17 kinds of amino acids content in yellow rice wine. OPA reagent was used for post-column derivatization and RP-HPLC gradient elution was operated before the determination. The determination results suggested that the linear correlation coefficient r was within 0.9990-0,9998, the average recovery was 94.5 %-98.7 %, and precision RSD was 0.51%- 1.90 %. Such method was highly specific and easy to operate with high sensitivity. It could be used for the determination of amino acids in yellow rice wine.

关 键 词：柱后衍生 反相高效液相色谱法 OPA试剂 氨基酸 黄酒 

分 类 号：TS262.4[轻工技术与工程—发酵工程] TS261.4[轻工技术与工程—食品科学与工程]