Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment:a radioimmunoconjugate for hepatocellular carcinoma imaging  

作　　者：Hui Jie Bian Zhi Nan Chen Jing Lan Deng 

机构地区：[1]Cell Engineering Research Center,Basic Medical.Department,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China [2]Department of Clinical Nuclear Medicine,Xijing Hospital,Xi’an 710033,Shaanxi Province,China

出　　处：《World Journal of Gastroenterology》2000年第3期348-352,共5页世界胃肠病学杂志（英文版）

基　　金：National Natural Science Foundation of China,No.39700175

摘　　要：AIM To directly radiolabel an anti-hepatomamAb fragment HAb18 F（ab’）₂ with ^99mTc bystannous-reduced method,and assess thestability,biodistribution and radioimmun-oimaging（RⅡ）.METHODS Immunoreactive fraction wasdetermined according to Lindmo’s method.Ellman’s reagent was used to determine thenumber of thiols in the reduced F（ab’）₂.Labelingefficiency and homogeneity were measured bypaper chromatography,sodium dodecylsulphatepolyacrylamide gel electrophoresis（SDS-PAGE）and autoradiography.Challenge assay involvedthe incubation of aliquots of labeled antibody inethylenediaminetetraacetate（ EDTA ）and L-cysteine（L-cys）solutions with different molarratio at 37℃ for 1h,respectively.Investigationsin vivo utilized nude mice bearing humanhepatocellular carcinoma（HHCC）xenograftswith gamma camera imaging and tissuebiodistribution studies at regular intervals.RESULTS The labeling procedure was finishedwithin 1.5 h compared with the'pretinning'method which would take at least 21h.In vitrostudies demonstrated that the radiolabeled mAbfragment was homogeneous and retained itsimmunoreactivity.Challenge studies indicatedthat ^99mTc-labeled HAb18 F（ab’）₂ in EDTA is morestable than in L-cys.Imaging and biodistribution showed a significant tumor uptake at 24 h post-injection of ^99mTc-labeled HAb18 F（ab’）₂.Theblood,kidney,liver and tumor uptakes at 24hwere 0.56±0.09,56.45±11.36,1.43±0.27 and6.57±3.01（%ID/g）,respectively.CONCLUSION ^99mTc-HAb18 F（ab’）₂ conjugateprepared by this direct method appears to be aneffective way to detect hepatoma in nude micemodel.AIM:To directly radiolabel an anti-hepatoma mAb fragment HAb18 F(ab')(2) with (99m)Tc by stannousreduced method, and assess the stability, biodistribution and radioimmun oimaging (R II).METHODS:Immunoreactive fraction was determined according to Lindmo's method. Ellman's reagent was used to determine the number of thiols in the reduced F(ab') (2). Labeling efficiency and homogeneity were measured by paper chromatography, sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. Challenge assay involved the incubation of aliquots of labeled antibody in ethylenediaminetetraacetate (EDTA) and L-cysteine (L-cys) solutions with different molar ratio at 37 degrees　for 1h, respectively. Investigations in vivo utilized nude mice bearing human hepatocellular carcinoma (HHCC) xenografts with gamma camera imaging and tissue biodistribution studies at regular intervals.RESULTS:The labeling procedure was finished within 1.5h compared with the pretinning method which would take at least 21h. In vitro studies demonstrated that the radiolabeled mAb fragment was homogeneous and retained its immunoreactivity. Challenge studies indicated that (99m)Tc-labeled HAb18 F(ab') (2) in EDTA is more stable than in L-cys. Imaging and biodistribution showed a significant tumor uptake at 24h post injection of (99m)Tc-labeled HAb18 F(ab') (2). The blood, kidney, liver and tumor uptakes at 24h were 0.56 +/- 0.09, 56.45 +/- 11.36,1.43 +/- 0.27 and 6.57 +/- 3.01 (%ID/g) respectively.CONCLUSION:(99m)Tc-HAb18 F(ab') (2) conjugate prepared by this direct method appears to be an effective way to detect hepatoma in nude mice model.

关 键 词：ANTIBODY MONOCLONAL ANTIBODY FRAGMENTS technetium-99m hepatocellular carcinoma liver neoplasms RADIOIMMUNOIMAGING 

分 类 号：R735.7[医药卫生—肿瘤]