INHIBITION OF C-MYC PROTEIN EXPRESSION AND CELL PROLIFERATION IN HL-60 CELLS BY ANTISENSE TRANSCRIPTS TO c-myc  

作　　者：郝秀娟[1] 

机构地区：[1]北京基础医学研究所,中化生物技术研究所,北京100850

出　　处：《中国实验血液学杂志》1995年第4期370-375,共6页Journal of Experimental Hematology

摘　　要：The human promyelocytic cell line HL-60 overexpresses the c-myc protooncogene. Plasmid pDACx carrying antisense human c-myc DNA and neo gene was introduced into HL-60 cells with lipofectin reagent. Upon DNA entering the tar-geted celis and expression of antisense transcripts to c-myc, C-MYC protein level, cell proliferation and colony-forming potentiality were all definitely inhibited.The human promyelocytic cell line HL-60 overexpresses the c-myc protooncogene. Plasmid pDACx carrying antisense human c-myc DNA and neo gene was introduced into HL-60 cells with lipofectin reagent. Upon DNA entering the tar-geted celis and expression of antisense transcripts to c-myc, C-MYC protein level, cell proliferation and colony-forming potentiality were all definitely inhibited.

关 键 词：C-MYC PROTOONCOGENE ANTISENSE TRANSCRIPT gene transfer 

分 类 号：R363[医药卫生—病理学]