ANALYSIS OF N-METHYL-N-NITROSOUREA-INDUCED MUTATIONS IN A SHUTTLE VECTOR PLASMID PROPAGATED IN MOUSE O^6-METHYLGUANINE-DNA METHYLTRANSFERASE-DEFICIENT CELLS IN COMPARISON WITH PROFICIENT CELLS.  

作　　者：Shin-ichiMoriwaki TakashiYagi ChikakoNishigori HirakuTakebe 

机构地区：[1]Faculty of Medicine,Kyoto University,Kyoto,Japan,Faculty of Medicine,Kyoto University,Kyoto,Japan,Faculty of Medicine,Kyoto University,Kyoto,Japan,Faculty of Medicine,Kyoto University,Kyoto,Japan

出　　处：《癌变．畸变．突变》1991年第S1期132-132,共1页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：To investigate a contribution of O-methylguanineto mutagenesis in mouse cells,we constructed ashuttle vector plasmid,pYZ289,from a part ofpZ189 plasmid and polyoma virus DNA.The plasmidcontains a supF gene as a marker of mutation andcan replicate in both E.coli and mouse cells.ThepYZ289 treated with N-methyl-N-nitrosourea (MNU)

关 键 词：PLASMID mutagenesis DNA PURINE marker mutation mutated GUANINE methy shuttle 

分 类 号：R73[医药卫生—肿瘤]