乳酸乳球菌Lactococcus lactis subsp.Lactis产丁二酮发酵条件研究  

Study on Butanedione Biosynthesis by Lactococcus Lactis Subsp. Lactis

作　　者：管世敏[1] 龚钢明[1] 顾佳伟[1] 

机构地区：[1]上海应用技术学院香精香料技术与工程学院,上海200235

出　　处：《食品工业》2011年第11期63-65,共3页The Food Industry

基　　金：上海应用技术学院引进人才青年基金(1020k103001-QJ2010-01);上海高校选拔培养优秀青年教师科研专项基金(405Zk10YQ-yyy10081)

摘　　要：对乳酸乳球菌乳亚种Lactococcus lactis subsp.Lactis发酵丁二酮条件进行研究,通过正交试验优化确定其最佳产丁二酮发酵条件为:温度37℃,起始pH6.5,接种量5%,柠檬酸钠的添加浓度0.75%。在此条件下,菌株在脱脂乳培养基中丁二酮的产量可达到69.58 mg/L。Through orthogonal experiments the optimum fermentation conditions of butanedione biosynthesis by Lactococcus lactis subsp.Lactis was determined.The best fermentation temperature was 37 ℃,the optimum fermentation was pH 6.5,the best inoculum was size 5%,the optimal concentration of sodium citrate was 0.75%.Under these conditions,the butanedione yield of strain Lactococcus lactis subsp.Lactis could be up to 69.58 mg/L in skim milk medium.

关 键 词：丁二酮 发酵 正交试验 

分 类 号：TQ224.24[化学工程—有机化工]