透性化处理提高黑曲霉细胞α-葡萄糖苷酶的表观转苷活力  

Permeabilization of Aspergillus niger for enhancing apparent transglucosylation activity of α-glucosidase

作　　者：陈桂光[1,2] 李玮[2,3] 符佳精[1,2] 张云开[1,2] 梁智群[1,2] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005 [2]广西亚热带生物资源保护利用重点实验室,广西南宁530004 [3]广西大学轻工与食品工程学院,广西南宁530004

出　　处：《食品工业科技》2011年第12期72-74,77,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(20362001);国家"863"项目(2006AA10Z339)

摘　　要：黑曲霉菌株D-597所产α-葡萄糖苷酶是胞内酶,完整细胞呈现的酶活力较低。研究了吐温-80、十六烷基溴化铵(CTAB)、乙醚、丙酮、戊二醛、乙醇等渗透剂的透性化效果,并对最适渗透剂戊二醛的透性化条件进行了优化,最优透性化处理条件为:每1g湿菌丝体添加30mL浓度为10%(v/v)的戊二醛溶液,处理温度30℃,处理时间60min。制备所得的透性化细胞的表观酶活达到483.9U/g湿菌体,是完整细胞表观酶活的193.3%。使用透性化细胞转化生产低聚异麦芽糖(IMO),其转化周期为24h,较完整细胞缩短24h,转化率保持在70%以上,具有一定的工业化应用前景。α-glucosidase was an intracellular enzyme in Aspergillus niger. The whole cells of Aspergillus niger exhibited low transglucosylation activity. Various chemical agents such as tween-80,cetyltrimethylammoinium bromide,diethyl ether,acetone,glutaral and ethanol were used to permeabilize the mycelium and demonstrated that glutaral was the most effective of them. The maximum transglucosylation activity（483. 9U/g wet cells,increased by 93. 3% compared to the whole cells）was obtained when cells were treated with 30mL 10%（v/v）glutaral per gram wet cells at 30℃ for 60min. The permeabilized Aspergillus niger cells convert liquefied cassava starch to isomaltooligosaccharides（IMO）within 24h（shorten by 24h than the whole cells）,the IMO yield accounted for 70%,demonstrating the research had great potential on IMO industrial production.

关 键 词：黑曲霉 低聚异麦芽糖 透性化 Α-葡萄糖苷酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]