胆盐水解酶基因bsh工程菌表达条件的优化  

Optimization of over-expression conditions of Lactobacillus plantarum bsh genes

作　　者：姚晶[1,2] 任婧[1] 吴正钧[1] 郭本恒[1,2] 

机构地区：[1]乳业生物技术国家重点实验室光明乳业股份有限公司,上海200436 [2]上海海洋大学食品学院,上海201306

出　　处：《浙江农业学报》2011年第6期1222-1226,共5页Acta Agriculturae Zhejiangensis

基　　金：国家科技部973计划(2010CB735705);上海市科委课题(09DZ2251400)

摘　　要：对来源于Lactobacillus plantarum ST-Ⅲ菌株的胆盐水解酶基因bsh1在工程菌E.coli BL21/pET28b-bsh1中的表达条件进行了优化。首先通过一系列单因素实验对影响工程菌发酵条件的因素水平进行筛选。然后将诱导培养时间、诱导剂异丙基硫代-β-D-半乳糖苷(isopropylthio-D-galactoside,IPTG)浓度、胆盐浓度、装液量4个影响因素进行正交试验。在最佳工艺条件下得到的胆盐水解酶BSH比酶活达817.73 U。The bsh1 gene was subcloned into expression vector pET28b and its product bile sale hydrolase（BSH） was over-expressed in Escherichia coli BL21.The appropriate value of each significant effect was noted with single factor experimental design.Effects of inducing time,isopropylthio-D-galactoside.IPTG concentration,bile salt concentration and amount of medium on BSH activity were investigated by orthogonal test.After orthogonal experimental design,under the optimal condition,the specific activity of BSH was 817.73 U.

关 键 词：胆盐水解酶 发酵条件 单因素实验 正交试验 

分 类 号：Q78[生物学—分子生物学]