超高压协同乳酸链球菌素抑制低温火腿中的耐压腐败菌  

Combined effect of ultra high pressure and Nisin on inactivation of spoilage pressure-resistant bacteria isolated from smoked cooked ham

作　　者：温斯颖[1] 韩衍青[2] 方东路[1] 陈威[1] 吴菊清[2] 徐幸莲[2] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]南京农业大学教育部肉品加工与质量控制重点实验室,江苏南京210095

出　　处：《食品工业科技》2012年第1期59-62,共4页Science and Technology of Food Industry

基　　金：国家大学生创新性实验计划课题(101030723);江苏省普通高校研究生创新计划项目(CX10B_310Z)

摘　　要：为探寻低温肉制品中耐压腐败菌的抑制手段,揭示超高压和乳酸链球菌素之间的协同抑菌效应。以肉制品中典型的耐压腐败菌绿色魏斯菌和肠膜明串珠菌为供试材料,评价二者在1～500μg/mL的乳酸链球菌素存在条件下的生长情况,并结合100～600MPa(5min,20℃)的超高压处理研究不同压力条件与乳酸链球菌素之间的协同增效作用。结果表明:随着乳酸链球菌素添加量的增加,两种菌的数量急剧降低,当添加量达到200μg/mL时,对二者的抑制程度达到最大;单独超高压处理时,绿色魏斯菌和肠膜明串珠菌分别在400MPa和300MPa的压力下受到显著抑制。而超高压结合200μg/mL乳酸链球菌素处理时,二者受到显著抑制的压力均降低到100MPa;500MPa,5min,20℃的超高压条件结合200μg/mL乳酸链球菌素处理能够抑制绿色魏斯菌数达9lg(cfu/mL)。超高压和乳酸链球菌素之间存在显著的协同作用,乳酸链球菌素的添加将会有效降低工业化生产中超高压的压力水平。The objective of this study was to evaluate the cooperate effect between high pressure and nisin content in inactivating pressure-resistant bacteria of cooked meat products.Typical spoilage pressure-resistant bacteria Weissella viridescens and Leuconostoc mesenteroides were treated with addition of 1～500μg/mL Nisin and also subjected to treatment at a range of high pressure from 100 to 600MPa（5min,20°C）.Standard culture methods were used to detecting the number of survivals.Results showed that,with the growing addition of nisin,numbers of the two bacteria were falling rapidly.When nisin content was up to 200μg/mL,these inactivate effect went to maximum.Significant reduction in bacteria numbers was found at high pressure treated at 400MPa and 300MPa for W.viridescens and L.mesenteroides,respectively.However,when high pressure treated with 200μg/mL Nisin,the pressure level fallen to 100MPa both for these two bacteria.High pressure treated at 500MPa for 5min at 20°C,with 200μg/mL Nisin content,could inactivate W.viridescens counts by 9lg（cfu/mL）.It provided evidence that there surely exist combined effect between high pressure and nisin when used for delaying bacteria grows.Nisin addition can effectively lower the pressure level used during industrial decontamination processes.

关 键 词：超高压 乳酸链球菌素 耐压菌 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]