微藻DHA油脂的酶法脱胶工艺研究  

Study on enzyme catalyzed degumming of micro-algae DHA oil

作　　者：陈园力[1] 胡耀池[1] 龚东平[1] 梁井瑞[2] 蒋露[2] 张红漫[2] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,江苏南京210009 [2]南京工业大学理学院,江苏南京210009

出　　处：《食品工业科技》2012年第1期223-225,229,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金重点项目(20936002);江苏省六大人才高峰项目(2008);江苏省高校科研成果产业化推进项目(2009)

摘　　要：采用嗜热真菌(Thermomyces lanuginosus)产磷脂酶A1对裂殖壶菌产DHA毛油进行脱胶处理,以脱胶油磷含量、酸价为考查指标,先对脱胶时间、反应温度、加酶量和加水量等因素进行单因素实验,然后通过正交实验得出微藻DHA油脂的最佳脱胶条件为:脱胶时间3h,反应温度45℃,加酶量0.6mL/100g油,加水量为2mL/100g油;此条件下油脂中磷脂含量从158.1mg/kg降到4.6mg/kg,酸价变化较小。与传统的脱胶工艺相比,新型酶法脱胶优势明显,具有良好的应用前景。Phospholipase A1 produced by Thermomyces lanuginosus was used for the DHA crude oil degumming which was fermented by Schizochytrium sp.Phosphorus content and acid value of the degumming oil was used as the indices.Firstly,the results of the reaction temperature and time,the dosage of adding water and enzyme was got through single factor test.Then through orthogonal test,the optimal conditions of micro-algae DHA oil enzymatic degumming were determined as follows：reaction time 3h,reaction temperature 45℃,enzyme dosage 0.6mL/100g crude oil,water dosage 2mL/100g crude oil.Under this condition of degumming,the phosphorus content was reduced from 158.1mg/kg to 6.4mg/kg,and acid value changed slightly.Compared with the hydration degumming process,new enzymatic degumming has many obvious advantages and good application prospects.

关 键 词：磷脂酶A1 酶法脱胶 DHA油脂 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]