检测乳粉中克罗诺杆菌属穆汀斯克罗诺杆菌的双抗夹心ELISA方法的研究  

Development of a double-antibody sandwich enzyme linked immunosorbent assay for detection of Cronobacter muytjensii in milk powder

作　　者：石曼[1] 生威[1] 杜欣军[1] 王帅[1] 杨丽[1] 余桂春[1] 郭柏雪[1] 王硕[1] 

机构地区：[1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品工业科技》2012年第1期335-337,共3页Science and Technology of Food Industry

基　　金：“十一五”科技支撑课题计划资助项目(2009BADB9B03,2009BADB9B06);天津市科技计划项目(10SYSYJC28300)

摘　　要：获得了抗穆汀斯克罗诺杆菌的多克隆抗体,抗体只对穆汀斯克罗诺杆菌菌株有特异性识别,而对非穆汀斯克罗诺杆菌无交叉反应。利用所得到的抗体,建立了一种双抗夹心ELISA检测方法,该方法对纯培养穆汀斯克罗诺杆菌菌液检出限为105cfu/mL;经过17h增菌,全脂乳粉染菌样品中的穆汀斯克罗诺杆菌的检出限为0.1cfu/g。该方法为快速检测乳粉中穆汀斯克罗诺杆菌的污染奠定了基础。The polyclonal antibody（PcAb） for Cronobacter muytjensii was available by intravenous injection with New Zealand rabbit with C.muytjensii ATCC51329 strains,a double-antibody sandwich enzyme-linked immunosorbent assay（ELISA） was developed.The detection limits were 105cfu/mL in pure culture of C.muytjensii ATCC51329 and without any cross reaction with the non-C.muytjensii strains.With 17h enrichment procedure,C.muytjensii recovered well from inoculated whole milk powder at levels of 0.1cfu/g.The results demonstrated that the double-antibody sandwich ELISA was a sensitive and specific method for detecting C.muytjensii in dairy products.

关 键 词：穆汀斯克罗诺杆菌 双抗夹心ELISA 全脂乳粉 检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]