茶油脱臭馏出物中甾醇精制的研究  

Study on the phytosterol extraction from oil-tea camellia seed oil deodorization distillate

作　　者：刘存存[1,2] 方学智[1] 李宝才[2] 王开良[1] 姚小华[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]昆明理工大学生命科学与技术学院,云南昆明650000

出　　处：《食品工业科技》2012年第2期250-253,256,共5页Science and Technology of Food Industry

基　　金：林业行业公益专项(2007-4-36);浙江省重点项目(2007C12G3030003)

摘　　要：采用乙醇萃取VE,皂化法提取茶油脱臭馏出物中甾醇化合物,以正己烷为结晶溶剂对甾醇化合物进行重结晶。VE最佳萃取参数:液料比3:1,搅拌时间30min,萃取温度55℃,搅拌速度80r/min。甾醇最佳的提取工艺条件:碱料比1(KOH):5,醇料比15(乙醇):1,皂化时间3h,温度85℃;最佳结晶工艺条件:液料比3:1,溶剂正己烷,结晶温度-2℃,结晶时间24h,三次重结晶后精制甾醇的纯度可以达到89.3%。Extracted VE from oil-tea camellia seed oil deodorization distillate by alcohol and gained phytosterols by saponification,the phytosterols were dissolved in n-hexane to recrystallize.The optimal conditions extraction of VE were liquor-raw material ratio 3： 1, back flow extract 30min, extraction temperature 55%, rate 80r/min.The optimal conditions extraction of phytosterol were as following.potassium hydroxide-raw material ratio 1 ：.5, ethanol-raw material ratio 15：1 ,and back flow saponified 3h under 85~C.The optimal crystallization conditions were as following= n-hexane- raw material ratio 3：1, temperature-2℃, time 24h, the refined phytosterols with 89.3% purity were obtained by three recrystallizations.

关 键 词：茶油 脱臭馏出物 VE 萃取 皂化 植物甾醇 重结晶 

分 类 号：TS225[轻工技术与工程—粮食、油脂及植物蛋白工程]