高效液相色谱-电感耦合等离子体质谱联用测定婴幼儿辅食中的无机砷  

Determination of Inorganic Arsenic in Complementary Foods for Infants and Young Children by HPLC-ICP/MS

作　　者：侯艳霞[1,2] 刘丽萍[1] 潘浩[3] 杜振霞[2] 李筱薇[3] 

机构地区：[1]北京市疾病预防控制中心,北京100013 [2]北京化工大学理学院,北京100029 [3]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《食品科学》2011年第24期281-284,共4页Food Science

摘　　要：目的:拟建立婴幼儿辅助食品中无机砷含量的测定方法。方法:以0.15mmol/L硝酸溶液为提取剂,90℃热浸提取婴幼儿辅助食品(米粉类及罐头类)中无机砷,采用Hamilton PRP-X100(250mm×4.1mm,10μm)色谱柱分离,建立高效液相色谱-电感耦合等离子体质谱(high performance liquid chromatography-inductively coupled plasmamass spectrometry,HPLC-ICP/MS)联用技术测定其含量的方法。结果表明:As(Ⅲ)与As(Ⅴ)的线性范围为0.5～400μg/L,线性相关系数(r)优于0.9990,检出限分别为0.06μg/L和0.08μg/L,无机砷的加标回收率在80.5%～1 28.5%之间,相对标准偏差(R SD)<5%。结论:方法简便、准确、灵敏,适用于婴幼儿辅助食品中无机砷含量的测定。Objectives： To establish a high performance liquid chromatography-inductively coupled plasma mass spectrometry method for the determination of inorganic arsenic in complementary food for infants and young children.Methods： Sample preparation was achieved by extraction with 0.15 mmol/L nitric acid at 90 ℃.Chromatographic separation was performed on a Hamilton PRP-X100 column（250 mm × 4.1 mm,10 μm）.Results： The linear ranges for As（Ⅲ） and As（Ⅴ） were both between 0.5 μg/L and 400 μg/L with a correlation coefficient of more than 0.9990.The detection limits for As（Ⅲ） and As（Ⅴ） were between 0.06 μg/L and 0.08 μg/L,and the spike recoveries were in the range of 80.5%-128.5% with a relative standard deviation（RSD） of less than 5%.Conclusions： This method was simple,sensitive and accurate,and could be used to determine the inorganic arsenic levels in complementary foods for infants young children.

关 键 词：高效液相色谱-电感耦合等离子体质谱联用技术 婴幼儿辅助食品 无机砷 测定 

分 类 号：TS216[轻工技术与工程—粮食、油脂及植物蛋白工程]