顶空固相微萃取-气质联用法测定樟芝菌丝体中挥发性化合物  

Analyze on Volatile Compounds of Antrodia camphorata Using HS-SPME-GC-MS

作　　者：何喆 陆震鸣 许泓瑜 史劲松 许正宏 

机构地区：[1]江南大学医药学院制药工程研究室,江苏无锡214122 [2]江南大学医药学院生物活性制品加工工程研究室,江苏无锡214122

出　　处：《中药材》2011年第11期1722-1725,共4页Journal of Chinese Medicinal Materials

基　　金：江苏省自然科学基金(BK2010142);国家"863"重点项目子课题(2007AA021506);教育部新世纪人才支持计划(NCET-07-0380)

摘　　要：目的:分析固体平板培养和液体深层培养的樟芝菌丝体中挥发性物质的组成。方法:利用顶空微萃取(HS-SPME)与气相色谱-质谱联用(GC-MS)的方法分析挥发性物质的组成。结果:分别在液体培养菌丝体和固体培养菌丝体中分析得到49种和43种化合物。其中,蘑菇醇、3-辛酮、蘑菇醇乙酸酯、乙酸辛酯和乙醇是液体培养樟芝菌丝体中主要的挥发性化合物。蘑菇醇、3-辛酮、3-甲基丁醛、γ-十二内酯、甲基-2-糠酸酯和蘑菇醇乙酸酯为固体培养樟芝菌丝体的主要挥发性化合物。结论:液体培养樟芝菌丝体和固体培养樟芝菌丝体的挥发性化合物组成相似,但各化合物的相对含量不同。Objective：To analyze the volatile compounds of Antrodia camphorata in solid-state and submerged cultures.Methods：A headspace solid-phase microextraction（HS-SPME） coupled with gaschromatography-mass spectrometry（GC-MS）were used to evaluate the profile of the volatile compounds.Results： 49 volatile compounds were identified in A.camphorata mycelia in submerged culture,while 43 volatile compounds were identified in mycelia in solid-state culture.1-octen-3-ol,3-octanone,1-octen-3-ylacetate,acetic acid octyl ester and ethanol were the main volatile compounds in A.camphorata mycelia in submerged culture,while 1-octen-3-ol,3-octanone,3-methyl-butyraldenhyde,γ-podecalactone and methyl 2-furozte were the most potent key volatile compounds in mycelia in solid-state culture.Conclusion：The volatile compounds in the mycelia of A.camphorata in solid-state and submerged cultures are similar but their relative contents are different.

关 键 词：樟芝 顶空固相微萃取 气相色谱-质谱 挥发性物质 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]