油茶皂甙的提取及抑菌活性研究  

Extraction and antibacterial activities of tea saponin

作　　者：唐红枫[1] 孙茜[2] 张雨婷[1] 戴立华[1] 王鸿焦[1] 程时劲[1] 

机构地区：[1]武汉东湖学院生命科学与化学学院,武汉430212 [2]湖北省疾病预防控制中心,武汉430079

出　　处：《中国油脂》2012年第2期69-71,共3页China Oils and Fats

基　　金：国家科技部国家科技支撑计划(2006BAJ02A10)

摘　　要：用溶剂法提取油茶饼中的油茶皂甙,经重结晶法制备的标准品标定有效成分含量为81.8%。以制备的油茶皂甙为材料对枯草杆菌、大肠杆菌、金黄色葡萄球菌进行抑菌实验。结果表明:油茶皂甙对这3种细菌均有抑制作用,且对枯草杆菌和金黄色葡萄球菌抑制作用较强,0.10 g/mL质量浓度油茶皂甙对3种细菌均具有致死作用;不同质量浓度油茶皂甙均能产生抑菌圈,抑菌圈直径大小与油茶皂甙浓度呈正相关。Tea saponin was extracted from oil -tea camellia seed cake with ethanol, and its antibacterial activities were studied. The results indicated that the tea saponin had restraining effects on Escherichia coli , Staphylococcus aureus and Bacillus subtilis , and its effects on Staphylococcus aureus and Bacillus subtilis were strong. The tea saponin with concentration of 0.10 g/mL had lethal effect on the three kinds of bacteria. The antibacterial circle diameter and the concentration of tea saponin were positively correlated.

关 键 词：油茶皂甙 提取 抑菌圈 抑菌活性 

分 类 号：TS201.2[轻工技术与工程—食品科学] R285.5[轻工技术与工程—食品科学与工程]