钛胶正相高效液相色谱法测定饮料中的山梨酸  

Determination of sorbic acid in beverages by titania-based high performance liquid chromatography

作　　者：钱瑞[1] 李荣[1] 谭津[1] 姜子涛[1] 

机构地区：[1]天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室,天津300134

出　　处：《中国食品添加剂》2012年第1期227-232,共6页China Food Additives

基　　金：国家自然科学基金项目(项目编号:20875069);天津商业大学青年基金项目(项目编号:090107)

摘　　要：防腐剂是能抑制微生物生长,防止食品腐败变质,延长保存期的一类添加剂。山梨酸在人体内能参加正常的新陈代谢,可视做食品的成分之一,是迄今为止国际公认的理想食品防腐剂。本文建立了根据钛胶正相高效液相色谱法测定饮料中防腐剂山梨酸含量的快速检测方法,4种饮料样品经过0.45μm微孔滤膜过滤后直接进样。色谱条件:分离柱为Titania Sachtopore-NP柱(250mm×4.6mm i.d.,5μm),流动相为水∶甲醇=20∶80(V/V)、流动相中含醋酸盐8mmol/L,流速0.8mL/min,柱温60℃,检测波长254nm。采用外标法定量,得到了良好的线性关系,线性范围为2-20μg/mL,检出限1.2μg/mL,回收率为98.2%103.9%,RSD(n=8)<0.74%。准确度和精密度均可满足饮料中山梨酸的测定要求,是一种简单、快速、结果准确的检测方法。Preservative is a kind of food additive which can improve the quality of food,extend the storage time and control the growth of microorganism.The sorbic acid can participate in human metabolism and thus are regarded as the component of the food.It has been recognized as the best preservative.A method for the determination of sorbic acid by HPLC（high performance liquid chromatography）on titania in beverage was reported.The samples were treated by 0.45μm millipore filter and separated with a Titania Sachtopore-NP column（250×4.6 mm,5μm）.The chromatographic separation conditions were as follows：a mixture of water and methanol in the ratio of 20 to 80 containing 8 mmol/L acetate used as mobile phase,flow rate of 0.8mL/min,column temperature 60℃,and the detection wavelength 254 nm.A good linear relationship is obtained by ESTD.The linear range of the method was 2-20μg/mL with a detection limit of 1.2 μg/mL.The recoveries were between 98.2% and 103.9%.RSD（n=8）was less than 0.74%.The method is applied to determine sorbic acid in beverage with satisfactory results.The method is simple,timesaving,and precise and it is applicable to the determination of aspartame in drinks.

关 键 词：山梨酸 钛胶柱 高效液相色谱法 饮料 

分 类 号：TS207.3[轻工技术与工程—食品科学]