纤溶豆豉的制备及其对小鼠溶栓效果的评价  

Preparation of Fibrinolytic Douchi and Evaluation of Its Thrombolytic Effectiveness in vivo

作　　者：李小东[1] 王成涛[1] 赵磊[1] 杨雪莲[1] 张佳婵[1] 

机构地区：[1]北京工商大学食品添加剂与配料北京高校工程研究中心,食品风味化学北京市重点实验室,北京100048

出　　处：《食品科学》2012年第3期228-231,共4页Food Science

基　　金：北京市属高校人才强教计划项目(PHR201008237);教育部-北京市教委科研重点项目(KM201110011001);学科与研究生教育-食品学科特色学科群建设项目(PXM2011-014213-113634)

摘　　要：利用动物血栓模型,饲喂纤溶豆豉来研究其在动物体内的溶栓作用。通过小鼠腰背部皮下注射10mg/mL角叉菜胶溶液诱发小鼠尾静脉形成血栓,实验组A饲喂豆豉纤溶豆豉并自由饮用纤溶豆豉提取液,对照组B(模型小鼠)、对照组C(正常小鼠)分别饲喂全价营养鼠粮,并自由饮水。结果表明:各组小鼠生长情况良好;饲喂纤溶豆豉的实验组A小鼠体质量先降低后增高,饲喂5d以后尾部形成的血栓长度逐渐变短,10d时凝血酶原时间、凝血酶时间稍有延长,但差异不显著,15d后凝血酶原时间、凝血酶时间均明显延长(P<0.05),血浆中纤维蛋白原(FIB)含量相应降低,D-二聚体在饲喂10d后呈阳性;而饲喂全价营养鼠粮的对照组B及注射生理盐水的对照组C,其P T、T T差异均不显著。因此食用纤溶豆豉能激活机体纤溶系统,抑制凝血系统作用,有助于预防和辅助治疗血栓性疾病。The effect of feeding Douchi was evaluated on mouse thrombosis induced by subcutaneous injection with 10 mg/mL carrageenan at the back waist of mice.ICR mice were divided into three groups： Douchi treatment group（thrombosis model mice fed Douchi and normal saline extract from Douchi）,model control group（thrombosis model mice fed full nutrition diet and distilled water） and normal control group（normal mice fed full nutrition diet and distilled water）.The results showed that the mice in the Douchi treatment group exhibited an initial decrease and subsequent increase in body weight,a slight increase in prothrombin time（PT） and thrombin time（TT）,and a reduction in plasma fibrinogen（FIB） during the experiment.The detection of D-dimer was positive after 10 days of feeding and PT and TT were notably prolonged（P 0.05） in the mice fed for 15 days.However,PT and TT showed no significant difference between the model control group and normal control group.Therefore,Douchi can activate the function of fibrinolysis,inhibit the function of blood-clotting system,and prevent and treat thrombosis diseases.

关 键 词：中国豆豉 小鼠血栓模型 血栓长度 纤溶活性 角叉菜胶 

分 类 号：TS201.4[轻工技术与工程—食品科学]