拉曼光谱研究脉冲电场作用下磷脂脂质体构象的变化  

Effect of pulsed electric fields on phospholipid vesicle conformation by Raman spectra

作　　者：贾晓[1] 曾新安[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品工业科技》2012年第5期138-140,共3页Science and Technology of Food Industry

基　　金：国家自然科学基金(21076088);国家"863项目"(2011AA100801);广东省国际合作重点项目(2009B050400003)

摘　　要：为深入研究脉冲电场的灭菌机理,探讨电场与细胞膜的作用机制,利用拉曼光谱谱图分析了不同电场场强下磷脂脂质体构象的变化。结果表明,拉曼峰值比I1085/I1064随着电场场强的增加而相应增大,而I1085/I1123随着电场场强的增加而先增加后减少,但比值仍高于空白样,说明经过电场处理后,脂质体C-C扭曲构象的数目增多;而I2885/I2850随着场强的增加呈现递减的趋势。由此可以说明经电场处理后的脂质体磷脂分子间烃链的无序度增加,导致细胞膜流动性增强。In order to study the sterilization mechanism of pulsed electric fields（PEF） on microorganisms and the interaction between PEF conditions and cell membrane composed of phospholipids,the phospholipid vesicle conformation changing under various electric field strength treatments was analyzed by Raman spectra.It was demonstrated from the Raman spectra that the intensity ratio of I1085/I1064 increased while I1085/I1123 increased firstly and then decreased later with increasing electric field strength,but the ratio was still higher than the control sample.It indicated a little increase of the gauche/trans population compared with control sample.On the other hand,the ratio of I2885/I2850 decreased with increasing electric field strength.The result showed that the intermolecular disordering in hydrocarbon chain of the lipid lattice increased and the membrane fluidity enhanced after being subjected PEF treatment.

关 键 词：拉曼光谱 脉冲电场 脂质体 构象 

分 类 号：TS201.22[轻工技术与工程—食品科学]