玉米低聚肽促HL-7702人肝细胞增殖作用  

Proliferation-promoting Effects of Corn Oligopeptides on Human Hepatocyte Line HL-7702 in vitro

作　　者：刘艳[1] 林峰[1] 金振涛[1] 陈亮[1] 易维学[1] 蔡木易[1] 

机构地区：[1]中国食品发酵工业研究院,北京100027

出　　处：《食品与发酵工业》2012年第2期43-46,共4页Food and Fermentation Industries

摘　　要：采用MTT法测定细胞增殖、流式细胞术分析细胞周期及细胞存活率,对玉米低聚肽的促HL-7702人肝细胞增殖作用进行了研究。结果显示:玉米低聚肽在1～10 g/L内,可促进肝细胞生长,其促进作用与肽浓度呈正相关;给予肝细胞玉米低聚肽24 h后,细胞周期中S期及G2/M期细胞的比例上升;并且在此浓度范围内,玉米低聚肽对肝细胞存活率及形态影响较小。研究表明,玉米低聚肽有促进HL-7702人肝细胞增殖及DNA合成的作用,可显著提高肝细胞活力,提示其在保肝护肝中可能具有一定作用。The proliferation-promoting effects of corn oligopeptides （COP） on HL-7702 cells in vitro were studied by MTT assay and flow cytometry（FCM）. Results indicated that COP could stimulate the growth of hepatocyte, and the promotion increased in a concentration-dependent manner over the range of 1 -10 g/L; the proportions of cells in S and G2/M phases of cell cycle were increased at 24h after the addition of COP; and in this concentration range, the influence of COP on cell viability and morphology were not significant. COP could enhance human hepatocyte line HL-7702 proliferation and DNA synthesis, and also can improve the vitality of hepatocyte. The study suggested that COP could have certain effect on liver protection.

关 键 词：玉米低聚肽 HL-7702人肝细胞 增殖 细胞周期 

分 类 号：R151[医药卫生—营养与食品卫生学]