采用RAPD-毛细管芯片电泳法鉴定啤酒大麦品系  

Identification of Malting Barley Using the RAPD-capillary Vesselchip Electrophoresis

作　　者：吴亚君[1] 王斌[1] 韩建勋[1] 张振民[2] 杨海荣[1] 陈颖[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]厦门出入境检验检疫局,福建厦门061012

出　　处：《食品与发酵工业》2012年第2期174-179,共6页Food and Fermentation Industries

基　　金：国家863计划(2011AA1008047);国家质检总局科技计划项目(2009IK316)

摘　　要：我国啤酒大麦主要依赖进口,建立快速准确的进口啤酒大麦品系鉴定方法对保护我国啤酒制造业利益,维护国际贸易公平具有重要意义。该实验采用RAPD和2100毛细管芯片电泳技术,建立了我国主要进口啤酒大麦品系鉴定方法。采用毛细管芯片电泳技术对11次重复实验数据进行统计分析,根据谱带重现性、特异性筛选标志带,根据标志带精确长度和相对量确定判别标准。5条随机引物产生的7条标志带将19个啤酒大麦品种分成13组。In China malting barley is mainly dependent on import. It is necessary to establish fast and accurate method for cuhivar identification to protect the benefit of brewery industry. In this paper RAPD and 2100 capillary vessel chip electrophoresis technique was applied to differentiate malting barley cultivars which were mainly used in brewery. Data of 11 repeated assays was statistically analyzed. The marker bands were selected according to reproducibility and specificity. Parameters of length and quantity of marker band were set. Seven marker bands produced by five random primers divided 19 malting barleys into 13 groups.

关 键 词：啤酒大麦 RAPD 毛细管芯片电泳 品系鉴定 

分 类 号：TS262.5[轻工技术与工程—发酵工程] O657.8[轻工技术与工程—食品科学与工程]