扇贝性腺多糖提取物的抗氧化及免疫调节活性  

Antioxidant and Immunomodulating Activities of Polysaccharide from Scallop Gonad

作　　者：宋荪阳[1,2] 孙黎明[1,2] 朱蓓薇[1,2] 牛海玲[1,2] 杨静峰[1,2] 

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]国家贝类加工技术研究分中心,辽宁大连116034

出　　处：《食品科学》2012年第5期248-251,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2008BAD94B07);辽宁省重点实验室建设项目(2008403003);辽宁省教育厅创新团队计划项目(2009T033;2008T007)

摘　　要：通过酶解法制备扇贝性腺多糖提取物(SGP),研究其抗体外氧化及免疫调节活性。采用1,1-二苯基-2-三硝基苯肼DPPH体系反应、Fenton反应和还原反应测定SGP的抗氧化活性;3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法测定SGP对活性氧叔丁基脂氢过氧化物(tBOOH)致细胞氧化损伤及淋巴细胞增殖活性的调节作用;分光光度法测定SGP对补体活性的影响。结果表明:SGP具有DPPH自由基清除能力(IC50=9.92mg/mL)和羟自由基清除能力(IC50=7.31mg/mL)及还原能力(AC20=7.74mg/mL);0.2μg/mL的SGP可明显改善tBOOH致RAW264.7细胞的氧化损伤作用,还可显著提高脾淋巴细胞的增殖活性及补体经典途径活性。Scallop gonad polysacchaxide exffact （SGP） was obtained by hydrolysis with a series of commercial enzymes. Its antioxidant activity was evaluated by DPPH system, Fenton system and reduction reaction. MTT assay was used to evaluate its effect on tBOOH-induced oxidative damage and lymphocyte proliferation. The effect on complement activity was evaluated by spectrophotometry. The results indicated that SGP had reducing power and scavenging effect on DPPH and hydroxyl free radicals. SGP at the 0.2 p g/mL could also protect RAW264.7 cells from oxidative damage induced by tBOOH. Moreover, SGP could also promote lymphocyte proliferation and activate complement activity.

关 键 词：扇贝性腺 多糖提取物 抗氧化 淋巴细胞增殖 补体 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]