浓香型大曲中微生物总DNA的PCR优化  

Optimization of PCR reaction system in Luzhou-flavor Daqu microbial DNA

作　　者：潘明[1,2] 袁城金[1] 王世宽[1,2] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]酿酒生物技术及应用四川省重点实验室,四川自贡643000

出　　处：《中国酿造》2012年第3期108-111,共4页China Brewing

基　　金：四川省教育厅重大培育项目(07ZZ018)

摘　　要：利用SDS-酶法、试剂盒法提取大曲总DNA,对粗提DNA梯度稀释稀释,同时按浓度梯度加入BSA进行PCR扩增。SDS-酶法提取总DNA的量是试剂盒法的300倍;对粗提DNA稀释50倍同时添加BSA浓度为0.6ng/μL的PCR扩增效果最佳。利用SDS-酶法能够最大量的提取曲药中总DNA,对总DNA进行稀释同时加入BSA能够有效的进行PCR扩增。The DNA in Daqu extracted by the SDS-enzymic technique and diagnostic method,was diluted with gradiented concentration,and finally was amplificated.The SDS-enzymic technique can extract 300 times larger amount DNA than the diagnostic method.The optimal amplification condition was that to didute 50 times,and add the BSA in a concentration of 0.6 ng/ul.It could extract plenty of total DNA by the SDS-enzymic technique,and it can amplificate the total DNA in a effective way when diluted the total DNA and add some BSA.

关 键 词：浓香型大曲 微生物 16S RDNA PCR扩增 

分 类 号：TS261.1[轻工技术与工程—发酵工程]