超高效液相色谱-串联四极杆质谱法快速筛查食品中的45种工业染料  

Rapid Screening of 45 Industrial Dyes in Foods by Ultra Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry

作　　者：路勇[1] 渠岩[2] 冯楠[1] 赵俊平[1] 金伟伟[2] 

机构地区：[1]北京市食品安全监控中心,北京100041 [2]北京市食品酿造研究所,北京100050

出　　处：《食品科学》2012年第6期176-180,共5页Food Science

基　　金：北京市科委计划项目(D101110047710004)

摘　　要：建立食品中45种工业染料残留量的超高效液相色谱-串联四极杆质谱快速筛查方法。样品经溶剂提取净化后,采用BEH C18液相色谱柱分离,以水和乙腈为流动相,进行梯度洗脱,采用多反应监测模式,外标法定量。结果表明,45种工业染料的方法定量限为0.1～100μg/kg;线性范围内低、中、高3个添加水平的回收率为61.51%～109.64%,相对标准偏差为5.23%～9.76%。该方法具有简便快捷、灵敏度高、结果准确等特点,适用于食品中上述工业染料的快速筛查工作。An ultra performance liquid chromatography-tandem quadrupole mass spectrometry（UPLC-MS/MS） method has been developed for the rapid screening of 45 industrial dyes residues in foods.Samples were extracted by solvent extraction and cleaned up before chromatographic separation on a BEH C18 column using a mobile phase consisting of water and acetonitrile by gradient elution.The dyes were analyzed by GC-MS/MS in multiple reaction monitoring（MRM） mode and quantified by the external standard method.The limits of detection for the dyes varied from 0.1 to 100μg/kg.The average recoveries across high,medium and low spike levels were between 61.51% and 109.64% with a relative standard deviation（RSD） in the range of 5.23%-9.76%.The method was simple,rapid,sensitive,accurate and suitable for use in the rapid screening of industrial dyes residues in foods.

关 键 词：工业染料 超高效液相色谱-串联质谱 食品 筛查 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657[轻工技术与工程—食品科学与工程]