番茄红素微囊生物利用度检测的实验研究  

Experimental study on bioavailability detection of lycopene microencapsulation

作　　者：龚秋翼[1] 钱俊青[1] 郭辉[1] 唐颖[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032

出　　处：《浙江工业大学学报》2012年第2期129-132,187,共5页Journal of Zhejiang University of Technology

基　　金：国家863计划基金资助项目(2007AA100403)

摘　　要：功能性食品制成微囊是一个前沿技术,但检测其生物利用度提高的方法较少,通过自制的番茄红素微囊,探索检测功能性食品微囊的生物利用度的方法.采用大鼠肠灌流实验,紫外分光光度法测定肠灌流液中番茄红素的浓度,依据药物在小肠段中的减少量来确定药物的吸收.制备得到粒径在(800±30)nm的番茄红素微囊,通过肠灌流实验,测得其吸收率由33.29%提高到58.34%,生物利用度提高.番茄红素微囊在整个小肠的吸收常数Ka为(0.39±0.08)h-1.番茄红素微胶囊化后在小肠的吸收率比番茄红素原料显著提高,吸收呈一级动力学.Microencapsulated functional foods could not only improve stability,but also benefit for the bioavailability of functional ingredients.It would be,desirable,therefore,to develop testing methods for the increase of its bioavailability.Through the self-made lycopene microencapsulation,intestinal perfusion experiments were used to evaluate the bioavailability.The concentrations of lycopene in the perfusate were determined by UV-spectrophotometric method.Based on the reduction of drug quantity in the small intestine to determine its absorption,preparation of lycopene microencapsulation with the particle size of（800±30） nm diameter,through the intestinal perfusion experiment for lycopene microencapsulation absorption in the small intestine were studied,the absorption was increased from the 33.29% to 58.34%.The bioavailability was significantly improved.Michaelis constant（Ka） of the active transport system was（0.28±0.03） h-1.The result suggested the absorption was a first-order process.

关 键 词：番茄红素 微胶囊 肠吸收 生物利用度 

分 类 号：TS264.4[轻工技术与工程—发酵工程]