高效液相色谱法测定老陈醋中的5-羟甲基糠醛  

Determination of 5-hydroxymethylfurfural(5-HMF)in over mature vinegar by HPLC

作　　者：周婷婷[1] 杨瑞学[2] 宋弋[1] 张玉玉[1] 吕茜[1] 倪元颖[1] 李全宏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业机械化科学研究院,北京100083

出　　处：《食品工业科技》2012年第8期83-85,共3页Science and Technology of Food Industry

基　　金："十二五"国家科技支撑计划项目(2011BAD23B01)

摘　　要：利用高效液相色谱法测定老陈醋中5-羟甲基糠醛(5-HMF)的含量,以5%甲醇水溶液为流动相,采用C18色谱柱,在284nm波长条件下检测样品中的5-HMF。结果表明,该方法快速、准确,在0.0143～0.1001μg范围内线性相关系数为R2=0.9994,平均回收率88.27%～96.69%,相对标准偏差为2.09%～4.70%。A method for the determination of 5-hydroxymethylfurfural by high-performance liquid chromatography in old mature vinegar was developed.Samples were analyzed on a Cls column at 284nm using a mixture of methanol and water（5：95, v/v）as the mobile phase.Within the linear range of 0.0143 - 0.1001μg , the method was accurate with the range of average recoveries rate and relative standard deviation of 88.27%-96.69% and 2.09% 4.70%, respectively.

关 键 词：老陈醋 高效液相色谱 5-羟甲基糠醛 

分 类 号：TS207.3[轻工技术与工程—食品科学]