硅胶柱层析分离啤酒花中的黄腐酚  

Separating xanthohumol from Munulus lupulus using silica gel column layer chromatography

作　　者：夏娜[1] 赵仲凯[1] 黄志江 魏洁麟[1] 徐效圣[3] 贾佳佳[1] 冯作山[1] 

机构地区：[1]新疆农业大学食品科学学院,乌鲁木齐830052 [2]新疆方睿啤酒原料制品有限责任公司,乌鲁木齐831300 [3]新疆轻工职业技术学院,乌鲁木齐830021

出　　处：《食品科技》2012年第4期174-177,181,共5页Food Science and Technology

基　　金：新疆维吾尔自治区科技攻关和重点项目计划项目(200731136-3)

摘　　要：利用硅胶柱层析法对啤酒花浸膏中的黄腐酚进行了分离,采取了2种洗脱方法。方法一是采用石油醚和乙酸乙酯固定比例(2:1)洗脱2次,第1次洗脱后合并含有黄腐酚的洗脱液,浓缩成浸膏,进行第2次洗脱;方法二是以石油醚和乙酸乙酯的混合溶剂梯度洗脱2次,第1次梯度洗脱后合并含有黄腐酚的洗脱液,浓缩成浸膏,进行第2次梯度洗脱。结果表明,方法一的分离效率高、速度快,黄腐酚得率较高(44.88%),但黄腐酚的纯度较低(57.78%);方法二的黄腐酚得率较低(43.16%),但纯度高(81.10%),部分馏分浓缩后有结晶析出,经HPLC检测为黄腐酚结晶单体,纯度达到98.1%,因此可以应用硅胶柱层析法高效分离和纯化啤酒花黄腐酚。Research was conducted using chromatography methods of silica gel column to purify xanthohumol from Munulus lupulus.Compared with the yield and purity of xanthohumol,two elution methods could be adopted,one of which is taking the petroleum ether：ethyl acetate（2：1）as the elution solvent,then,condense the output and do the same elution process.The other way is taking the mixed petroleum ether and ethyl acetate with different proportion as the elution solvent to do gradient elution,after that,mix the output with the solvent,do the same gradient elution.The result shows that：the first method is of higher efficiency,speed,and yield（44.88%）,but the purity of xanthohumol is lower（57.78 %）.In the second method,the yield of xanthohumol is lower（43.16%）,but the purity of xanthohumol is higher（81.10%）.Some of the output educed xanthohumol crystallization.This study determined the xanthohumol crystallization with the HPLC,the purity reach up to 98.1%.Thus,chromatography method of silica gel column is adoptable to purify the xanthohumol from Munulus lupulus.

关 键 词：硅胶 柱层析 啤酒花 黄腐酚 

分 类 号：R284.2[医药卫生—中药学]