胞壁溶解酶用于黑木耳破壁提取工艺的研究  

Optimization of the Extraction Conditions of Polysaccharide from Auricularia Auricula with Wall-breaking Enzyme

作　　者：鲜乔[1] 张拥军[1] 蒋家新[1] 朱丽云[1] 李佳[1] 谢亚君[1] 

机构地区：[1]中国计量学院生命科学学院,杭州310018

出　　处：《中国食品学报》2012年第3期96-103,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省科技创新团队项目(2010R50028)

摘　　要：为更好的开发利用黑木耳,提取其多糖,借助minitab统计软件,采用中心组合试验法对破壁酶提取黑木耳多糖的工艺条件进行优化,并在相同条件下,比较该酶与其它提取方法的多糖得率。结果表明,以提取温度、提取时间、液料比三因素为自变量,以多糖提取率为因变量,回归得到二次多项式模型,结果显著,拟合情况良好。模型校正决定系数为0.9989,相关系数为0.9979。通过对回归方程进行局部寻优分析,得到黑木耳多糖的最佳酶提工艺条件为:添加量400U/g,pH8.0,提取温度42℃、提取时间1.65h,液料比98mL/g。此条件下多糖提取率达11.78%,显著高于其他提取方法。The conditions were optimized to extract the polysaccharides from Auricularia auricular with wall-braking enzyme using central combination testing method. The extraction yield using this method was higher than other. The muhinomial models ~were ~obtained using temperature, extraction time, and the ratio of fluid to material as independent variables and polysaccharide extraction rate as dependent variable.The model reached a determination coefficient of 0.9989 with the correlation coefficient of 0.9979. The partial optimization analysis to the regression equation indicated that the optimal technological conditions for polysaccharide extraction from Auricularia auricula with the wall-breking enzyme were as follows： the extraction temperature of 42 ℃, the extraction time of 1.65 h, and ratio of fluid to material of 98 mL/g with 400 U/g recruitment and pH 8.0. Under the optimal conditions, the extraction rate reached as high as 11.78%.

关 键 词：黑木耳 多糖 提取工艺 优化 

分 类 号：TQ461[化学工程—制药化工]