辅助能量物质柠檬酸促进ε-聚赖氨酸的合成  

Enhancement of ε-PL production with Supplement of Auxiliary Energy Source Citric Acid by Streptomyces sp.M-Z18

作　　者：任喜东[1] 陈旭升[1] 董难[1] 李树[1] 李凤[1] 赵福林 唐蕾[1] 毛忠贵[1] 

机构地区：[1]江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡214122 [2]无锡市世纪生物药业有限公司,江苏无锡214092

出　　处：《食品与发酵工业》2012年第3期23-27,共5页Food and Fermentation Industries

基　　金：无锡市科技支撑项目(CYE21N1107)

摘　　要：为提高ε-聚赖氨酸(ε-PL)合成能力,考察了柠檬酸对Streptomyces sp.M-Z18菌体生长和ε-PL合成的影响。研究发现,柠檬酸作为辅助能量物质,对ε-PL发酵过程影响显著。通过对柠檬酸添加时间、添加量和pH值的优化,确定了最佳柠檬酸添加方式,结合补料-分批发酵工艺,显著提高了ε-PL的产量。实验结果表明,在5L发酵罐中,维持pH为3.8,初始柠檬酸添加量为20 g/L时,分批发酵ε-PL的产量达到9.50 g/L,产率达到4.40 g/(L.d),较未添加柠檬酸发酵分别提高了46.4%和48.1%。采用添加柠檬酸的补料-分批发酵工艺,发酵168 h后ε-PL的产量达到22.89 g/L,是分批发酵的2.41倍。The synthesis of ε-PL was performed by adding citric acid as auxiliary energy material.The results showed that contribution of citric acid to the fermentation of ε-PL was obvious.The optimum dose,addition time of citric acid and batch fermentations at various pH values were studied.Under the optimized condition,pH 3.8 and 20 g/L citric acid,the production of ε-PL reached 9.50 g/L and the productivity of ε-PL was 4.40 g/（L·d）,it was enhanced by 46.4% and 48.1%,respectively,in contrast with original batch fermentation strategy.Moreover,the fed-batch production of ε-PL reached 22.89 g/L,2.41-fold of that in batch fermentation.

关 键 词：Ε-聚赖氨酸 STREPTOMYCES sp.M-Z18 柠檬酸 补料-分批发酵 

分 类 号：TQ922.3[轻工技术与工程—发酵工程]