絮凝-沉淀法制备油茶皂素工艺研究  

Preparation of camellia saponin by flocculation-precipitation method

作　　者：王金元[1] 费学谦[1] 罗凡[1] 陈焱[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《中国油脂》2012年第4期60-64,共5页China Oils and Fats

基　　金："948"引进项目(2009-4-56);国家"十一五"科技支撑课题(2009BADB1B03)

摘　　要：以水酶法制取油茶籽油后的水相为原料,采用絮凝-沉淀法提取其中的油茶皂素。单因素实验得出适宜的絮凝条件为:1%的壳聚糖溶液加入量为水相体积的16%,此时油茶皂素的损失率为22.24%。应用正交实验确定沉淀-转化的最佳工艺为:在絮凝离心分离后的水溶液中,加入质量3%的氧化钙,在20℃下沉淀6 h,离心后,加入转化剂碳酸氢铵(加入量为2.5倍的氧化钙量),40℃下释放0.5 h,最终得到纯度为80.25%的油茶皂素。此法在提高产品纯度的同时,浓缩了油茶皂素水溶液,降低了能耗。The aqueous phase obtained in the oil -tea camellia seeds oil extraction by aqueous enzymatic method was used as raw material, and the camellia saponin was extracted by flocculation -precipitation method. Based on the single factor experiments, it was found to be better when 1% chitosan solution was used with the amount of 16% total liquid volume and the loss rate of camellia saponin was 22. 24%. The supernatant obtained by flocculation - centrifuge was used as material, and the orthogonal test was em- ployed to confirm the optimal precipitation -transformation conditions. The optimal conditions were deter- mined as follows： the amount of CaO 3% （on the basis of the supernatant mass）was added to the super- natant and then precipitated for 6 h at 20℃; after centrifugation, NH4HCO3 was added to the sediment and its dosage was 2.5 times of CaO amount, and then released at 40℃ for 0. 5 h. Under the optimal conditions the content of camellia saponin was 80. 25 %. This method could increase the product purity as well as concentrating the camellia saponin aqueous solution, and reduce the energy consumption.

关 键 词：油茶皂素 絮凝 沉淀 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ423[轻工技术与工程—食品科学与工程]