甜酒曲中产糖化酶根霉菌株紫外诱变选育  

Breeding of a Glucoamylase-Producing Strain of Rhizopus from Sweet Rice Wine Starter by Ultraviolet Radiation

作　　者：邓开野[1] 林佩芳[1] 黎尔纳[1] 

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510225

出　　处：《食品科学》2012年第7期204-208,共5页Food Science

摘　　要：从甜酒曲中分离出产糖化酶根霉菌株,通过平板菌落初筛、测定HE值和摇瓶复筛测糖化力,筛选出1株HE值大且糖化力高的优良出发菌株,对其进行紫外线诱变处理,选育得到优良变异株,该菌株的HE值为1.76,糖化力为1281mg/(h.g),分别较出发菌株高出5.4%、59.5%。对此优良变异株的培养条件进行优化,获得最佳的培养条件为:培养温度为30℃,培养时间为3d,添加玉米粉质量浓度为8g/100mL,在此条件下糖化力为1317mg/(h.g)。An excellent strain of Rhizopus with high HE value and saccharification power from sweet rice starter was screened through flat plate primary screening and mutagenized by UV radiation. An excellent mutant strain with an HE value of 1.76 and a sacchariflcation power of 1281 mg/（h· g） was obtained, which were increased by 5.4% and 59.5% compared to those of the original strain, respectively. The optimal culture conditions for the mutant strain were culture temperature of 30 ℃, culture time of 3 days and corn flour concentration of 8 g/100 mL. Under the optimal conditions, the sacchariflcation power of the screened strain was up to 1317 mg/（h · g）.

关 键 词：甜酒曲 根霉 紫外线诱变 糖化力 

分 类 号：Q933[生物学—微生物学]