类芽孢杆菌产胆固醇氧化酶的发酵条件优化  

Optimization of fermentation conditions of cholesterol oxidase produced by Paenibacillus sp X534

作　　者：赵芳[1] 张建[1] 杨林华[1] 周河治[2] 

机构地区：[1]邯郸职业技术学院管理系,河北邯郸056001 [2]广西大学生命科学与技术学院,广西南宁530004

出　　处：《中国酿造》2012年第4期130-133,共4页China Brewing

基　　金：广西壮族自治区自然科学基金项目(桂科自0728017)

摘　　要：对诱变过的产胆固醇氧化酶的类芽孢杆菌(Paenibacillus sp X534)进行了发酵条件的优化,确定其最适培养基为:蔗糖2%,大豆粉2%,MgSO4.7H2O 0.1%,MnSO40.02%,土豆汁30%,K2HPO4.3H2O 0.4%,Tween-80 0.1%。确定最适发酵条件为:起始pH值为8,接种量8%,装液量16%,摇瓶转速180r/min,培养温度34℃,发酵时间50h。优化之后胆固醇氧化酶活达到400U/L左右,比优化前提高了约2倍。Fermentation conditions optimization for cholesterol oxidase production with Paenibacillus sp X534,which was mutated and selected,was studied.The optimal culture medium was： sucrose 2%,soybean powder 2%,MgSO4·7H2O 0.1%,MnSO4 0.02%,patato juice 30%,K2HPO4·3H2O 0.4%,Tween-80 0.1%.The optimal culture conditions were as follows,initial pH value 8.0,inoculum 8%,filling rate 40mL medium/250mL flask,rotation speed 180r/min,cultured 50h at 34℃.Under the optimized cultivation conditions,the enzyme activity reached 400U/L,which was 2 times higher than that of origin conditions.

关 键 词：胆固醇氧化酶 发酵 优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]