两种常见固定化脂肪酶的酶学性质和微观结构比较  

Study on the enzyme properties and microstructure of two immobilized lipase

作　　者：陈晓慧[1] 李越[1] 时敏[1] 李志平[1] 于殿宇[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2012年第10期216-218,222,共4页Science and Technology of Food Industry

基　　金：国家863高技术研究发展计划(2010AA101503)

摘　　要：分别对游离脂肪酶、大孔树脂吸附-交联固定化脂肪酶和海藻酸钠固定化脂肪酶的热稳定性、pH稳定性和操作稳定性等方面进行研究。结果显示,固定化脂肪酶在热稳定性、pH稳定性和重复利用次数上均有明显提高。对大孔树脂吸附-交联和海藻酸钠固定化微球进行了表观结构的考察。从SEM图片可以看出:海藻酸钠固定化微球表面和内部为均匀的蜂窝状多孔性结构,孔径较大,约0.1mm;而大孔树脂吸附-交联固定化微球内部具有规则蜂窝状通孔结构,孔径都在几微米以下。说明大孔树脂吸附-交联固定化脂肪酶效果比较理想。The thermal stability,pH stability and operational stability and so on of free lipase,lipase immobilized by macroporous resin and sodium alginate were studied separately.The results indicated that the thermal stability,pH stability and reutilization frequency of immobilized lipase were markedly improved.The apparent structure of macroporous resin and sodium alginate microcapsule were inspected respectively by SEM.It could be seen from those pictures：Honeycomb and cavities were distributed uniformly on the surface and in the interior of calcium alginate microcapsule,larger diameter was 0.1mm or so.The internal structure of macroporous resin microcapsule was rules-hole,and aperture which was less than few microns.Thus,immobilized lipase by macroporous resin was better.

关 键 词：固定化 脂肪酶 SEM 酶活 孔径 

分 类 号：TS201.25[轻工技术与工程—食品科学]