水相体系酶法制备甘油磷脂酰胆碱的研究  

Preparation of glyceryl phosphoryl choline catalyzed by Thermo4S-3

作　　者：张康逸[1] 陈岩[1] 刘元法[1] 王兴国[1] 

机构地区：[1]江南大学食品学院,食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品工业科技》2012年第10期219-222,共4页Science and Technology of Food Industry

基　　金：国家863高技术研究发展计划(2010AA101506)

摘　　要：研究了脂肪酶(Thermo4S-3)在水相体系中制备甘油磷脂酰胆碱(GPC)的可行性,并探讨了脂肪酶水解条件对GPC得率的影响。实验结果表明脂肪酶(Thermo4S-3)可以实现豆粉末磷脂的水解反应,具有制备甘油磷酸胆碱(GPC)的工业应用前景。正交实验优化结果如下:反应温度40℃,底物浓度33.3g/L,酶添加量30U/mL,CaCl2添加量2g/L。利用HPLC-ELSD进行定量分析,GPC得率达到96.5%。LC-MS/MS定性分析,GPC的结构与标样分析的一致。Thermo4S-3 was applied to prepare of glyceryl phosphoryl choline（GPC） in aqueous media.The effects of reaction parameters on the yield of GPC were discussed.The optimum conditions were as follows：Temperature 40℃,substrate concentration 33.3g/L,Thermo4S-3 loading 30U/mL,dosage of CaCl2 2g/L.The final GPC was determined by HPLC-ELSD with the yield 96.5%.The results of LC-MS/MS showed that the structure of L-α-GPC was agreement with that of standard sample.The results indicated that our study provided a feasible method for synthesis of GPC.

关 键 词：磷脂酰胆碱 甘油磷脂酰胆碱 脂肪酶(Thermo4S-3) 酶解反应 

分 类 号：TS201.2[轻工技术与工程—食品科学]