固定化葡萄糖异构酶活化条件对其酶活的影响  

Influence of activation conditions on the enzyme activity of immobilized glucose isomerase

作　　者：胡弢[1] 周雪艳[1] 赵国群[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《食品工业科技》2012年第10期223-225,共3页Science and Technology of Food Industry

摘　　要：固定化葡萄糖异构酶在使用前需先进行活化,从而使酶发挥其最佳催化功效。本文从糖液浓度、温度、pH、活化时间和金属离子五个方面研究了活化条件对GENSWEETTMIGI-SA固定化葡萄糖异构酶酶活的影响。该酶的最适活化条件为:葡萄糖液浓度60%、温度55℃、pH7.5、时间4h。经此条件活化之后,其酶活达815U/g,与常规活化条件相比,酶活提高了40%以上。固定化葡萄糖异构酶活化时不宜加入Mg2+、Co2+、Mn2+和Zn2+。Activation was need before immobilized glucose isomerase was used in order to make it have the best catalytic ability.The influences of activation conditions on the enzyme activity of GENSWEETTM IGI-SA immobilized glucose isomerase were studied including concentration of glucose syrup,temperature,pH,activation time and metal ions.The optimal activation condition was as follows：Glucose syrup 60%,temperature 55℃,pH 7.5 and activation time 4h.Under this condition,the enzyme activity of immobilized glucose isomerase was 815U/g,which was 40% higher than one under normal activation condition.Mg2＋,Co2＋,Mn2＋and Zn2＋were not necessary to be added into glucose syrup when immobilized glucose isomerase was activated.

关 键 词：活化 果葡糖浆 固定化葡萄糖异构酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]