北京洋槐蜜化学成分及其抗氧化活性  

Chemical Components and Antioxidant Activity of Acacia Honey from Beijing

作　　者：孙丽萍[1] 穆雪峰[1,2] 施海燕 徐响[1] 庞杰[2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]福建农林大学食品科学学院,福建福州350002 [3]北京百花蜂业科技发展股份公司,北京100176

出　　处：《食品科学》2012年第9期77-80,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD33B04);国家现代农业(蜂)产业技术体系建设专项(CARS-45-KXJ18);科技部农业科技成果转化资金项目(2009GB23260456)

摘　　要：采用层析法富集北京洋槐蜜中抗氧化成分,利用高效液相测定其化学组成并研究其中已知化学成分对DPPH自由基的清除作用,旨在评价北京洋槐蜜的抗氧化功能。结果显示:北京洋槐蜜中含有绿原酸、咖啡酸、对香豆酸、山奈酚、异鼠李素、松属素、白杨素和高良姜素8种已知成分,含量在9.5～257.8μg/100g之间,除松属素和白杨素外,其余各成分均有清除DPPH自由基的能力,且差异显著(P<0.01)。从有效成含量及抗氧化能力综合考虑,山奈酚可视为北京洋槐蜜中最有代表性的抗氧化成分。Anfioxidant compounds from Acacia honey from Beijing were enriched by column chromatography and detected by high-performance liquid chromatography. The antioxidant activity of these compounds was evaluated by DPPH radicalscavenging assay. Eight compounds were detected in Acacia honey, which were chlorogenic acid, caffeic acid, p-coumaric acid, kaempferol, isorhamnetin, pinocembrin, chrysin and galangin at the content range of 9.5 -- 257.8 μ g/100 g. Meanwhile, a significant difference in DPPH radical-scavenging capacity was also observed. In terms of both the content and antioxidant capacity, kaempferol could be recognized as the most representative antioxidant component of Acacia honey from Beijing.

关 键 词：洋槐蜜 化学成分 抗氧化活性 DPPH自由基 山奈酚 

分 类 号：S896.1[农业科学—特种经济动物饲养]