杜仲叶绿原酸提取物与绿原酸、维生素C体外抗氧化比较  

Comparative study on the anti-oxidative ability of chlorogenic acid extract in leaves of Eucommia ulmoides, chlorogenic acid and vitamine C

作　　者：李文娜[1] 肖苑[1] 陈阳[1] 刘银花[1] 

机构地区：[1]遵义医学院珠海校区药理学教研室,广东珠海519041

出　　处：《食品工业科技》2012年第11期137-140,共4页Science and Technology of Food Industry

基　　金：遵义医学院"博士启动基金"项目;贵州省中医药管理局项目(QZYY-2010-60)

摘　　要：目的:比较杜仲叶绿原酸提取物(ECE)与绿原酸和维生素C(VC)的体外抗氧化活性。方法:以VC、绿原酸为对照,测定ECE对二苯代苦味酰肼自由基(DPPH.)、超氧阴离子自由基(O2-.)、羟基自由基(.OH)、烷基自由基的清除率及抑制大鼠红细胞氧化溶血的作用。结果:ECE对DPPH.、O2-.、.OH和烷基自由基的清除能力较好,其清除上述自由基的IC50值分别为14.0、38.3、61.1、648.7μg/mL,均小于绿原酸和VC的相应值,但ECE对红细胞氧化溶血的抑制效果不如绿原酸和VC。结论:ECE可作为多功能的天然抗氧化剂,值得进一步开发利用。Objective=To compare the antioxidant capacities of chlorogenic acid extract in leaves of Eucommia ulmoides（ECE）,chlorogenic acid and vitamine C（Vc） in vitro. Methods=The antioxidant effects of three agents were studied with five different experiments in vitro. They were the eliminating tests for 1,1-diphenyl 2- picrylhydrazyl （DPPH）,superoxide anion free radicals （02-·）, hydroxyl free radicals （· OH）,and alkane free radicals,as well as the inhibiting test for rats erythrocyte hemolysis. Results：The ECE had stronger effect on scavenging DPPH- ,O2-. OH and alkane free radicals,its IC values for eliminating above free radicals were 14.0,38.3,61.1 and 648.7pg/mL,respectively,which were less than the corresponding values for chlorogenic acid and hemolysis. However,the inhibition capacity to the hemolysis of red blood cells was lower than chlorogenic acid and hemolysis. Conclusion The ECE can be used as raw materials of natural radical scavenger.

关 键 词：杜仲叶 绿原酸 维生素C 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]